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C0818, a novel curcumin derivative, interacts with Hsp90 and inhibits Hsp90 ATPase activity
The aims of the present study were to estimate the affinity between 3,5-(E)-bis(3-methoxy-4-hydroxybenzal)-4-piperidinone hydrochloride (C0818) and heat shock protein 90 (Hsp90) and to investigate the inhibitory effects of this compound on Hsp90 ATPase activity. Fluorescence spectroscopy was used to...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5237709/ https://www.ncbi.nlm.nih.gov/pubmed/28119813 http://dx.doi.org/10.1016/j.apsb.2016.05.014 |
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author | Fan, Yingjuan Liu, Yang Zhang, Lianru Cai, Fang Zhu, Liping Xu, Jianhua |
author_facet | Fan, Yingjuan Liu, Yang Zhang, Lianru Cai, Fang Zhu, Liping Xu, Jianhua |
author_sort | Fan, Yingjuan |
collection | PubMed |
description | The aims of the present study were to estimate the affinity between 3,5-(E)-bis(3-methoxy-4-hydroxybenzal)-4-piperidinone hydrochloride (C0818) and heat shock protein 90 (Hsp90) and to investigate the inhibitory effects of this compound on Hsp90 ATPase activity. Fluorescence spectroscopy was used to examine the affinity between varying concentrations of C0818 and Hsp90, N-Hsp90, M-Hsp90 and C-Hsp90. Fluorescence intensities were recorded in the range of 290–510 nm at 293, 303 and 310 K, respectively. A colorimetric assay for inorganic phosphate (based on the formation of a phosphomolybdate complex and the subsequent reaction with malachite green) were used to examine the inhibitory effects of C0818 on Hsp90 ATPase activity. The equilibrium dissociation constant K(D) value of C0818 was found to be 23.412±0.943 μmol/L. The interaction between C0818 and Hsp90 was driven mainly by electrostatic interactions. C0818 showed the strongest affinity with C-Hsp90. These results conclusively demonstrate the inhibitory activity of C0818 on the activity of Hsp90 ATPase. |
format | Online Article Text |
id | pubmed-5237709 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-52377092017-01-24 C0818, a novel curcumin derivative, interacts with Hsp90 and inhibits Hsp90 ATPase activity Fan, Yingjuan Liu, Yang Zhang, Lianru Cai, Fang Zhu, Liping Xu, Jianhua Acta Pharm Sin B Original Article The aims of the present study were to estimate the affinity between 3,5-(E)-bis(3-methoxy-4-hydroxybenzal)-4-piperidinone hydrochloride (C0818) and heat shock protein 90 (Hsp90) and to investigate the inhibitory effects of this compound on Hsp90 ATPase activity. Fluorescence spectroscopy was used to examine the affinity between varying concentrations of C0818 and Hsp90, N-Hsp90, M-Hsp90 and C-Hsp90. Fluorescence intensities were recorded in the range of 290–510 nm at 293, 303 and 310 K, respectively. A colorimetric assay for inorganic phosphate (based on the formation of a phosphomolybdate complex and the subsequent reaction with malachite green) were used to examine the inhibitory effects of C0818 on Hsp90 ATPase activity. The equilibrium dissociation constant K(D) value of C0818 was found to be 23.412±0.943 μmol/L. The interaction between C0818 and Hsp90 was driven mainly by electrostatic interactions. C0818 showed the strongest affinity with C-Hsp90. These results conclusively demonstrate the inhibitory activity of C0818 on the activity of Hsp90 ATPase. Elsevier 2017-01 2016-06-18 /pmc/articles/PMC5237709/ /pubmed/28119813 http://dx.doi.org/10.1016/j.apsb.2016.05.014 Text en © 2017 Chinese Pharmaceutical Association and Institute of Materia Medica, Chinese Academy of Medical Sciences. Production and hosting by Elsevier B.V. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Original Article Fan, Yingjuan Liu, Yang Zhang, Lianru Cai, Fang Zhu, Liping Xu, Jianhua C0818, a novel curcumin derivative, interacts with Hsp90 and inhibits Hsp90 ATPase activity |
title | C0818, a novel curcumin derivative, interacts with Hsp90 and inhibits Hsp90 ATPase activity |
title_full | C0818, a novel curcumin derivative, interacts with Hsp90 and inhibits Hsp90 ATPase activity |
title_fullStr | C0818, a novel curcumin derivative, interacts with Hsp90 and inhibits Hsp90 ATPase activity |
title_full_unstemmed | C0818, a novel curcumin derivative, interacts with Hsp90 and inhibits Hsp90 ATPase activity |
title_short | C0818, a novel curcumin derivative, interacts with Hsp90 and inhibits Hsp90 ATPase activity |
title_sort | c0818, a novel curcumin derivative, interacts with hsp90 and inhibits hsp90 atpase activity |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5237709/ https://www.ncbi.nlm.nih.gov/pubmed/28119813 http://dx.doi.org/10.1016/j.apsb.2016.05.014 |
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