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Topoisomerase I inhibition leads to length-dependent gene expression changes in human primary astrocytes

Topoisomerase I is required for the proper expression of long genes (> 100 kb) in mouse and human cortical neurons, including many candidate genes for autism spectrum disorder (ASD) [1]. Given the important role of astrocytes in brain development [2], we investigated whether long genes, including...

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Autores principales: Gokoolparsadh, Akira, Fang, Zhiming, Braidy, Nady, Voineagu, Irina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5238604/
https://www.ncbi.nlm.nih.gov/pubmed/28119819
http://dx.doi.org/10.1016/j.gdata.2016.12.005
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author Gokoolparsadh, Akira
Fang, Zhiming
Braidy, Nady
Voineagu, Irina
author_facet Gokoolparsadh, Akira
Fang, Zhiming
Braidy, Nady
Voineagu, Irina
author_sort Gokoolparsadh, Akira
collection PubMed
description Topoisomerase I is required for the proper expression of long genes (> 100 kb) in mouse and human cortical neurons, including many candidate genes for autism spectrum disorder (ASD) [1]. Given the important role of astrocytes in brain development [2], we investigated whether long genes, including autism susceptibility genes, also require topoisomerase I expression in human primary astrocytes. We carried genome-wide expression profiling of cultured human primary astrocytes following treatment with the topoisomerase I inhibitor Topotecan, using Illumina microarrays. We identified several thousands of differentially expressed genes and confirmed that topoisomerase I inhibition affects gene expression in human primary astrocytes in a length-dependent manner. We also identified over 20 ASD-associated genes that show topoisomerase-dependent gene expression in human primary astrocytes but have not been previously reported as topoisomerase-I-dependent in neurons. The microarray data have been deposited in NCBI GEO (https://www.ncbi.nlm.nih.gov/geo/) under accession number GSE90052.
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spelling pubmed-52386042017-01-24 Topoisomerase I inhibition leads to length-dependent gene expression changes in human primary astrocytes Gokoolparsadh, Akira Fang, Zhiming Braidy, Nady Voineagu, Irina Genom Data Data in Brief Topoisomerase I is required for the proper expression of long genes (> 100 kb) in mouse and human cortical neurons, including many candidate genes for autism spectrum disorder (ASD) [1]. Given the important role of astrocytes in brain development [2], we investigated whether long genes, including autism susceptibility genes, also require topoisomerase I expression in human primary astrocytes. We carried genome-wide expression profiling of cultured human primary astrocytes following treatment with the topoisomerase I inhibitor Topotecan, using Illumina microarrays. We identified several thousands of differentially expressed genes and confirmed that topoisomerase I inhibition affects gene expression in human primary astrocytes in a length-dependent manner. We also identified over 20 ASD-associated genes that show topoisomerase-dependent gene expression in human primary astrocytes but have not been previously reported as topoisomerase-I-dependent in neurons. The microarray data have been deposited in NCBI GEO (https://www.ncbi.nlm.nih.gov/geo/) under accession number GSE90052. Elsevier 2016-12-23 /pmc/articles/PMC5238604/ /pubmed/28119819 http://dx.doi.org/10.1016/j.gdata.2016.12.005 Text en © 2016 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Data in Brief
Gokoolparsadh, Akira
Fang, Zhiming
Braidy, Nady
Voineagu, Irina
Topoisomerase I inhibition leads to length-dependent gene expression changes in human primary astrocytes
title Topoisomerase I inhibition leads to length-dependent gene expression changes in human primary astrocytes
title_full Topoisomerase I inhibition leads to length-dependent gene expression changes in human primary astrocytes
title_fullStr Topoisomerase I inhibition leads to length-dependent gene expression changes in human primary astrocytes
title_full_unstemmed Topoisomerase I inhibition leads to length-dependent gene expression changes in human primary astrocytes
title_short Topoisomerase I inhibition leads to length-dependent gene expression changes in human primary astrocytes
title_sort topoisomerase i inhibition leads to length-dependent gene expression changes in human primary astrocytes
topic Data in Brief
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5238604/
https://www.ncbi.nlm.nih.gov/pubmed/28119819
http://dx.doi.org/10.1016/j.gdata.2016.12.005
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