Cargando…
Topoisomerase I inhibition leads to length-dependent gene expression changes in human primary astrocytes
Topoisomerase I is required for the proper expression of long genes (> 100 kb) in mouse and human cortical neurons, including many candidate genes for autism spectrum disorder (ASD) [1]. Given the important role of astrocytes in brain development [2], we investigated whether long genes, including...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2016
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5238604/ https://www.ncbi.nlm.nih.gov/pubmed/28119819 http://dx.doi.org/10.1016/j.gdata.2016.12.005 |
_version_ | 1782495735356325888 |
---|---|
author | Gokoolparsadh, Akira Fang, Zhiming Braidy, Nady Voineagu, Irina |
author_facet | Gokoolparsadh, Akira Fang, Zhiming Braidy, Nady Voineagu, Irina |
author_sort | Gokoolparsadh, Akira |
collection | PubMed |
description | Topoisomerase I is required for the proper expression of long genes (> 100 kb) in mouse and human cortical neurons, including many candidate genes for autism spectrum disorder (ASD) [1]. Given the important role of astrocytes in brain development [2], we investigated whether long genes, including autism susceptibility genes, also require topoisomerase I expression in human primary astrocytes. We carried genome-wide expression profiling of cultured human primary astrocytes following treatment with the topoisomerase I inhibitor Topotecan, using Illumina microarrays. We identified several thousands of differentially expressed genes and confirmed that topoisomerase I inhibition affects gene expression in human primary astrocytes in a length-dependent manner. We also identified over 20 ASD-associated genes that show topoisomerase-dependent gene expression in human primary astrocytes but have not been previously reported as topoisomerase-I-dependent in neurons. The microarray data have been deposited in NCBI GEO (https://www.ncbi.nlm.nih.gov/geo/) under accession number GSE90052. |
format | Online Article Text |
id | pubmed-5238604 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-52386042017-01-24 Topoisomerase I inhibition leads to length-dependent gene expression changes in human primary astrocytes Gokoolparsadh, Akira Fang, Zhiming Braidy, Nady Voineagu, Irina Genom Data Data in Brief Topoisomerase I is required for the proper expression of long genes (> 100 kb) in mouse and human cortical neurons, including many candidate genes for autism spectrum disorder (ASD) [1]. Given the important role of astrocytes in brain development [2], we investigated whether long genes, including autism susceptibility genes, also require topoisomerase I expression in human primary astrocytes. We carried genome-wide expression profiling of cultured human primary astrocytes following treatment with the topoisomerase I inhibitor Topotecan, using Illumina microarrays. We identified several thousands of differentially expressed genes and confirmed that topoisomerase I inhibition affects gene expression in human primary astrocytes in a length-dependent manner. We also identified over 20 ASD-associated genes that show topoisomerase-dependent gene expression in human primary astrocytes but have not been previously reported as topoisomerase-I-dependent in neurons. The microarray data have been deposited in NCBI GEO (https://www.ncbi.nlm.nih.gov/geo/) under accession number GSE90052. Elsevier 2016-12-23 /pmc/articles/PMC5238604/ /pubmed/28119819 http://dx.doi.org/10.1016/j.gdata.2016.12.005 Text en © 2016 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Data in Brief Gokoolparsadh, Akira Fang, Zhiming Braidy, Nady Voineagu, Irina Topoisomerase I inhibition leads to length-dependent gene expression changes in human primary astrocytes |
title | Topoisomerase I inhibition leads to length-dependent gene expression changes in human primary astrocytes |
title_full | Topoisomerase I inhibition leads to length-dependent gene expression changes in human primary astrocytes |
title_fullStr | Topoisomerase I inhibition leads to length-dependent gene expression changes in human primary astrocytes |
title_full_unstemmed | Topoisomerase I inhibition leads to length-dependent gene expression changes in human primary astrocytes |
title_short | Topoisomerase I inhibition leads to length-dependent gene expression changes in human primary astrocytes |
title_sort | topoisomerase i inhibition leads to length-dependent gene expression changes in human primary astrocytes |
topic | Data in Brief |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5238604/ https://www.ncbi.nlm.nih.gov/pubmed/28119819 http://dx.doi.org/10.1016/j.gdata.2016.12.005 |
work_keys_str_mv | AT gokoolparsadhakira topoisomeraseiinhibitionleadstolengthdependentgeneexpressionchangesinhumanprimaryastrocytes AT fangzhiming topoisomeraseiinhibitionleadstolengthdependentgeneexpressionchangesinhumanprimaryastrocytes AT braidynady topoisomeraseiinhibitionleadstolengthdependentgeneexpressionchangesinhumanprimaryastrocytes AT voineaguirina topoisomeraseiinhibitionleadstolengthdependentgeneexpressionchangesinhumanprimaryastrocytes |