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Efficient in situ detection of mRNAs using the Chlorella virus DNA ligase for padlock probe ligation

Padlock probes are single-stranded DNA molecules that are circularized upon hybridization to their target sequence by a DNA ligase. In the following, the circulated padlock probes are amplified and detected with fluorescently labeled probes complementary to the amplification product. The hallmark of...

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Detalles Bibliográficos
Autores principales: Schneider, Nils, Meier, Matthias
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory Press 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5238799/
https://www.ncbi.nlm.nih.gov/pubmed/27879431
http://dx.doi.org/10.1261/rna.057836.116