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Efficient in situ detection of mRNAs using the Chlorella virus DNA ligase for padlock probe ligation
Padlock probes are single-stranded DNA molecules that are circularized upon hybridization to their target sequence by a DNA ligase. In the following, the circulated padlock probes are amplified and detected with fluorescently labeled probes complementary to the amplification product. The hallmark of...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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Cold Spring Harbor Laboratory Press
2017
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5238799/ https://www.ncbi.nlm.nih.gov/pubmed/27879431 http://dx.doi.org/10.1261/rna.057836.116 |