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Epsin2 promotes polarity establishment and meiotic division through activating Cdc42 in mouse oocyte
Epsins are a conserved family of endocytic adaptors essential for diverse biological events. However, its role in oocytes remains completely unknown. Here, we report that specific depletion of Epsin2 in mouse oocytes significantly disrupts meiotic progression. Confocal microscopy reveals that Epsin2...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Impact Journals LLC
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5239448/ https://www.ncbi.nlm.nih.gov/pubmed/27463009 http://dx.doi.org/10.18632/oncotarget.10815 |
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author | Li, Ling Han, Longsen Zhang, Jiaqi Liu, Xiaohui Ma, Rujun Hou, Xiaojing Ge, Juan Wang, Qiang |
author_facet | Li, Ling Han, Longsen Zhang, Jiaqi Liu, Xiaohui Ma, Rujun Hou, Xiaojing Ge, Juan Wang, Qiang |
author_sort | Li, Ling |
collection | PubMed |
description | Epsins are a conserved family of endocytic adaptors essential for diverse biological events. However, its role in oocytes remains completely unknown. Here, we report that specific depletion of Epsin2 in mouse oocytes significantly disrupts meiotic progression. Confocal microscopy reveals that Epsin2 knockdown results in the failure of actin cap formation and polar body extrusion during meiosis, indicative of the importance of Epsin2 in polarity establishment and cytokinesis. In addition, spindle defects and chromosome misalignment are readily observed in oocytes depleted of Epsin2. Moreover, we find that Epsin2 knockdown markedly decreases the activity of Cdc42 in oocytes and importantly, that the dominant-positive mutant of Cdc42 (Cdc42Q61L) is capable of partially rescuing the deficient phenotypes of Epsin2-knockdown oocytes. Together, our data identify Epsin2 as a novel player in regulating oocyte maturation, and demonstrate that Epsin2 promotes polarity establishment and meiotic division via activating Cdc42. |
format | Online Article Text |
id | pubmed-5239448 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Impact Journals LLC |
record_format | MEDLINE/PubMed |
spelling | pubmed-52394482017-01-24 Epsin2 promotes polarity establishment and meiotic division through activating Cdc42 in mouse oocyte Li, Ling Han, Longsen Zhang, Jiaqi Liu, Xiaohui Ma, Rujun Hou, Xiaojing Ge, Juan Wang, Qiang Oncotarget Research Paper: Pathology Epsins are a conserved family of endocytic adaptors essential for diverse biological events. However, its role in oocytes remains completely unknown. Here, we report that specific depletion of Epsin2 in mouse oocytes significantly disrupts meiotic progression. Confocal microscopy reveals that Epsin2 knockdown results in the failure of actin cap formation and polar body extrusion during meiosis, indicative of the importance of Epsin2 in polarity establishment and cytokinesis. In addition, spindle defects and chromosome misalignment are readily observed in oocytes depleted of Epsin2. Moreover, we find that Epsin2 knockdown markedly decreases the activity of Cdc42 in oocytes and importantly, that the dominant-positive mutant of Cdc42 (Cdc42Q61L) is capable of partially rescuing the deficient phenotypes of Epsin2-knockdown oocytes. Together, our data identify Epsin2 as a novel player in regulating oocyte maturation, and demonstrate that Epsin2 promotes polarity establishment and meiotic division via activating Cdc42. Impact Journals LLC 2016-07-24 /pmc/articles/PMC5239448/ /pubmed/27463009 http://dx.doi.org/10.18632/oncotarget.10815 Text en Copyright: © 2016 Li et al. http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Paper: Pathology Li, Ling Han, Longsen Zhang, Jiaqi Liu, Xiaohui Ma, Rujun Hou, Xiaojing Ge, Juan Wang, Qiang Epsin2 promotes polarity establishment and meiotic division through activating Cdc42 in mouse oocyte |
title | Epsin2 promotes polarity establishment and meiotic division through activating Cdc42 in mouse oocyte |
title_full | Epsin2 promotes polarity establishment and meiotic division through activating Cdc42 in mouse oocyte |
title_fullStr | Epsin2 promotes polarity establishment and meiotic division through activating Cdc42 in mouse oocyte |
title_full_unstemmed | Epsin2 promotes polarity establishment and meiotic division through activating Cdc42 in mouse oocyte |
title_short | Epsin2 promotes polarity establishment and meiotic division through activating Cdc42 in mouse oocyte |
title_sort | epsin2 promotes polarity establishment and meiotic division through activating cdc42 in mouse oocyte |
topic | Research Paper: Pathology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5239448/ https://www.ncbi.nlm.nih.gov/pubmed/27463009 http://dx.doi.org/10.18632/oncotarget.10815 |
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