New Insights into Cooperative Binding of Homeodomain Transcription Factors PREP1 and PBX1 to DNA

PREP1 and PBX1 are homeodomain (HD) transcription factors that play crucial roles in embryonic development. Here, we present the first biophysical characterization of a PREP1 HD, and the NMR spectroscopic study of its DNA binding pocket. The data show that residues flanking the HD participate in DNA...

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Autores principales: Zucchelli, Chiara, Ferrari, Elena, Blasi, Francesco, Musco, Giovanna, Bruckmann, Chiara
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5240567/
https://www.ncbi.nlm.nih.gov/pubmed/28094776
http://dx.doi.org/10.1038/srep40665
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author Zucchelli, Chiara
Ferrari, Elena
Blasi, Francesco
Musco, Giovanna
Bruckmann, Chiara
author_facet Zucchelli, Chiara
Ferrari, Elena
Blasi, Francesco
Musco, Giovanna
Bruckmann, Chiara
author_sort Zucchelli, Chiara
collection PubMed
description PREP1 and PBX1 are homeodomain (HD) transcription factors that play crucial roles in embryonic development. Here, we present the first biophysical characterization of a PREP1 HD, and the NMR spectroscopic study of its DNA binding pocket. The data show that residues flanking the HD participate in DNA binding. The kinetic parameters for DNA binding of individual PREP1 and PBX1 HDs, and of their combination, show that isolated PREP1 and PBX1 HDs bind to DNA in a cooperative manner. A novel PREP1 motif, flanking the HD at the C-terminus, is required for cooperativity.
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spelling pubmed-52405672017-01-23 New Insights into Cooperative Binding of Homeodomain Transcription Factors PREP1 and PBX1 to DNA Zucchelli, Chiara Ferrari, Elena Blasi, Francesco Musco, Giovanna Bruckmann, Chiara Sci Rep Article PREP1 and PBX1 are homeodomain (HD) transcription factors that play crucial roles in embryonic development. Here, we present the first biophysical characterization of a PREP1 HD, and the NMR spectroscopic study of its DNA binding pocket. The data show that residues flanking the HD participate in DNA binding. The kinetic parameters for DNA binding of individual PREP1 and PBX1 HDs, and of their combination, show that isolated PREP1 and PBX1 HDs bind to DNA in a cooperative manner. A novel PREP1 motif, flanking the HD at the C-terminus, is required for cooperativity. Nature Publishing Group 2017-01-17 /pmc/articles/PMC5240567/ /pubmed/28094776 http://dx.doi.org/10.1038/srep40665 Text en Copyright © 2017, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Zucchelli, Chiara
Ferrari, Elena
Blasi, Francesco
Musco, Giovanna
Bruckmann, Chiara
New Insights into Cooperative Binding of Homeodomain Transcription Factors PREP1 and PBX1 to DNA
title New Insights into Cooperative Binding of Homeodomain Transcription Factors PREP1 and PBX1 to DNA
title_full New Insights into Cooperative Binding of Homeodomain Transcription Factors PREP1 and PBX1 to DNA
title_fullStr New Insights into Cooperative Binding of Homeodomain Transcription Factors PREP1 and PBX1 to DNA
title_full_unstemmed New Insights into Cooperative Binding of Homeodomain Transcription Factors PREP1 and PBX1 to DNA
title_short New Insights into Cooperative Binding of Homeodomain Transcription Factors PREP1 and PBX1 to DNA
title_sort new insights into cooperative binding of homeodomain transcription factors prep1 and pbx1 to dna
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5240567/
https://www.ncbi.nlm.nih.gov/pubmed/28094776
http://dx.doi.org/10.1038/srep40665
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