A method for rapidly screening functionality of actin mutants and tagged actins

Recombinant production and biochemical analysis of actin mutants has been hampered by the fact that actin has an absolute requirement for the eukaryotic chaperone CCT to reach its native state. We therefore have developed a method to rapidly screen the folding capacity and functionality of actin var...

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Detalles Bibliográficos
Autores principales: Rommelaere, Heidi, Waterschoot, Davy, Neirynck, Katrien, Vandekerckhove, Joël, Ampe, Christophe
Formato: Texto
Lenguaje:English
Publicado: Biological Procedures Online 2004
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC524212/
https://www.ncbi.nlm.nih.gov/pubmed/15514698
http://dx.doi.org/10.1251/bpo94

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC524212/
https://www.ncbi.nlm.nih.gov/pubmed/15514698
http://dx.doi.org/10.1251/bpo94

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