Robust, universal biomarker assay to detect senescent cells in biological specimens

Cellular senescence contributes to organismal development, aging, and diverse pathologies, yet available assays to detect senescent cells remain unsatisfactory. Here, we designed and synthesized a lipophilic, biotin‐linked Sudan Black B (SBB) analogue suitable for sensitive and specific, antibody‐en...

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Detalles Bibliográficos
Autores principales: Evangelou, Konstantinos, Lougiakis, Nikolaos, Rizou, Sophia V., Kotsinas, Athanassios, Kletsas, Dimitris, Muñoz‐Espín, Daniel, Kastrinakis, Nikolaos G, Pouli, Nicole, Marakos, Panagiotis, Townsend, Paul, Serrano, Manuel, Bartek, Jiri, Gorgoulis, Vassilis G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2016
Materias:
Short Take
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5242262/
https://www.ncbi.nlm.nih.gov/pubmed/28165661
http://dx.doi.org/10.1111/acel.12545
Descripción
Sumario:Cellular senescence contributes to organismal development, aging, and diverse pathologies, yet available assays to detect senescent cells remain unsatisfactory. Here, we designed and synthesized a lipophilic, biotin‐linked Sudan Black B (SBB) analogue suitable for sensitive and specific, antibody‐enhanced detection of lipofuscin‐containing senescent cells in any biological material. This new hybrid histo‐/immunochemical method is easy to perform, reliable, and universally applicable to assess senescence in biomedicine, from cancer research to gerontology.

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