Optimized CRISPR-Cas9 Genome Editing for Leishmania and Its Use To Target a Multigene Family, Induce Chromosomal Translocation, and Study DNA Break Repair Mechanisms

CRISPR-Cas9-mediated genome editing has recently been adapted for Leishmania spp. parasites, the causative agents of human leishmaniasis. We have optimized this genome-editing tool by selecting for cells with CRISPR-Cas9 activity through cotargeting the miltefosine transporter gene; mutation of this...

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Detalles Bibliográficos
Autores principales: Zhang, Wen-Wei, Lypaczewski, Patrick, Matlashewski, Greg
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2017
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5244264/
https://www.ncbi.nlm.nih.gov/pubmed/28124028
http://dx.doi.org/10.1128/mSphere.00340-16

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5244264/
https://www.ncbi.nlm.nih.gov/pubmed/28124028
http://dx.doi.org/10.1128/mSphere.00340-16

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