Evaluation of sense-strand mRNA amplification by comparative quantitative PCR

BACKGROUND: RNA amplification is required for incorporating laser-capture microdissection techniques into microarray assays. However, standard oligonucleotide microarrays contain sense-strand probes, so traditional T7 amplification schemes producing anti-sense RNA are not appropriate for hybridizati...

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Detalles Bibliográficos
Autores principales: Goff, Loyal A, Bowers, Jessica, Schwalm, Jaime, Howerton, Kevin, Getts, Robert C, Hart, Ronald P
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2004
Materias:
Methodology Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC524485/
https://www.ncbi.nlm.nih.gov/pubmed/15469607
http://dx.doi.org/10.1186/1471-2164-5-76

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC524485/
https://www.ncbi.nlm.nih.gov/pubmed/15469607
http://dx.doi.org/10.1186/1471-2164-5-76

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