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Optimisation of methods for bacterial skin microbiome investigation: primer selection and comparison of the 454 versus MiSeq platform

BACKGROUND: The composition of the skin microbiome is predicted to play a role in the development of conditions such as atopic eczema and psoriasis. 16S rRNA gene sequencing allows the investigation of bacterial microbiota. A significant challenge in this field is development of cost effective high...

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Detalles Bibliográficos
Autores principales: Castelino, Madhura, Eyre, Stephen, Moat, John, Fox, Graeme, Martin, Paul, Ho, Pauline, Upton, Mathew, Barton, Anne
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5251215/
https://www.ncbi.nlm.nih.gov/pubmed/28109256
http://dx.doi.org/10.1186/s12866-017-0927-4