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Superior Multiplexing Capacity of PlexPrimers Enables Sensitive and Specific Detection of SNPs and Clustered Mutations in qPCR
BACKGROUND: Whilst qPCR provides an extremely powerful tool for genetic analysis, some applications such as multiplexing variant alleles (eg SNPs, point mutations or deletions), remain challenging using current primer/probe systems. The novel design features of PlexPrimers allow sensitive, multiplex...
Autores principales: | Tan, Lit Yeen, Walker, Samantha Michelle, Lonergan, Tina, Lima, Nicole Elizabeth, Todd, Alison Velyian, Mokany, Elisa |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5256879/ https://www.ncbi.nlm.nih.gov/pubmed/28114309 http://dx.doi.org/10.1371/journal.pone.0170087 |
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