Comparative evaluation of rRNA depletion procedures for the improved analysis of bacterial biofilm and mixed pathogen culture transcriptomes

Global transcriptomic analysis via RNA-seq is often hampered by the high abundance of ribosomal (r)RNA in bacterial cells. To remove rRNA and enrich coding sequences, subtractive hybridization procedures have become the approach of choice prior to RNA-seq, with their efficiency varying in a manner d...

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Detalles Bibliográficos
Autores principales: Petrova, Olga E., Garcia-Alcalde, Fernando, Zampaloni, Claudia, Sauer, Karin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5259769/
https://www.ncbi.nlm.nih.gov/pubmed/28117413
http://dx.doi.org/10.1038/srep41114

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5259769/
https://www.ncbi.nlm.nih.gov/pubmed/28117413
http://dx.doi.org/10.1038/srep41114

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