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IL-1β induces apoptosis and autophagy via mitochondria pathway in human degenerative nucleus pulposus cells

IL-1β has been reported highly expressed in degenerative intervertebral disc, and our previous study indicated IL-1β facilitates apoptosis of human degenerative nucleus pulposus (NP) cell. However, the underlying molecular mechanism remains unclear. We here demonstrate that IL-1β played a significan...

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Autores principales: Shen, Jieliang, Xu, Shengxi, Zhou, Hao, Liu, Huzhe, Jiang, Wei, Hao, Jie, Hu, Zhenming
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5264394/
https://www.ncbi.nlm.nih.gov/pubmed/28120948
http://dx.doi.org/10.1038/srep41067
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author Shen, Jieliang
Xu, Shengxi
Zhou, Hao
Liu, Huzhe
Jiang, Wei
Hao, Jie
Hu, Zhenming
author_facet Shen, Jieliang
Xu, Shengxi
Zhou, Hao
Liu, Huzhe
Jiang, Wei
Hao, Jie
Hu, Zhenming
author_sort Shen, Jieliang
collection PubMed
description IL-1β has been reported highly expressed in degenerative intervertebral disc, and our previous study indicated IL-1β facilitates apoptosis of human degenerative nucleus pulposus (NP) cell. However, the underlying molecular mechanism remains unclear. We here demonstrate that IL-1β played a significantly pro-apoptotic effect under serum deprivation. IL-1β decreased Bcl-2/Bax ratio and enhanced cytochrome C released from mitochondria to cytosol, which proved mitochondria-meidated apoptosis was induced. Subsequently, mitochondria damage was detected under IL-1β stimualtion. In addition, IL-1β-mediated injuried mitochondria contributes to activate autophagy. However, pretreatment with the autophagy inhibitor 3-methyladenine showed the potential in further elevating the apoptosis rate induced by IL-1β in NP cells. Our results indicated that the mitochondrial pathway was involved in IL-1β-induced apoptosis of NP cells. Meanwhile, the damaged mitochondria-induced autophagy played a protective role against apoptosis, suggesting a postive feedback mechanism under inflammatory stress.
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spelling pubmed-52643942017-01-30 IL-1β induces apoptosis and autophagy via mitochondria pathway in human degenerative nucleus pulposus cells Shen, Jieliang Xu, Shengxi Zhou, Hao Liu, Huzhe Jiang, Wei Hao, Jie Hu, Zhenming Sci Rep Article IL-1β has been reported highly expressed in degenerative intervertebral disc, and our previous study indicated IL-1β facilitates apoptosis of human degenerative nucleus pulposus (NP) cell. However, the underlying molecular mechanism remains unclear. We here demonstrate that IL-1β played a significantly pro-apoptotic effect under serum deprivation. IL-1β decreased Bcl-2/Bax ratio and enhanced cytochrome C released from mitochondria to cytosol, which proved mitochondria-meidated apoptosis was induced. Subsequently, mitochondria damage was detected under IL-1β stimualtion. In addition, IL-1β-mediated injuried mitochondria contributes to activate autophagy. However, pretreatment with the autophagy inhibitor 3-methyladenine showed the potential in further elevating the apoptosis rate induced by IL-1β in NP cells. Our results indicated that the mitochondrial pathway was involved in IL-1β-induced apoptosis of NP cells. Meanwhile, the damaged mitochondria-induced autophagy played a protective role against apoptosis, suggesting a postive feedback mechanism under inflammatory stress. Nature Publishing Group 2017-01-25 /pmc/articles/PMC5264394/ /pubmed/28120948 http://dx.doi.org/10.1038/srep41067 Text en Copyright © 2017, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Shen, Jieliang
Xu, Shengxi
Zhou, Hao
Liu, Huzhe
Jiang, Wei
Hao, Jie
Hu, Zhenming
IL-1β induces apoptosis and autophagy via mitochondria pathway in human degenerative nucleus pulposus cells
title IL-1β induces apoptosis and autophagy via mitochondria pathway in human degenerative nucleus pulposus cells
title_full IL-1β induces apoptosis and autophagy via mitochondria pathway in human degenerative nucleus pulposus cells
title_fullStr IL-1β induces apoptosis and autophagy via mitochondria pathway in human degenerative nucleus pulposus cells
title_full_unstemmed IL-1β induces apoptosis and autophagy via mitochondria pathway in human degenerative nucleus pulposus cells
title_short IL-1β induces apoptosis and autophagy via mitochondria pathway in human degenerative nucleus pulposus cells
title_sort il-1β induces apoptosis and autophagy via mitochondria pathway in human degenerative nucleus pulposus cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5264394/
https://www.ncbi.nlm.nih.gov/pubmed/28120948
http://dx.doi.org/10.1038/srep41067
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