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IL-1β induces apoptosis and autophagy via mitochondria pathway in human degenerative nucleus pulposus cells
IL-1β has been reported highly expressed in degenerative intervertebral disc, and our previous study indicated IL-1β facilitates apoptosis of human degenerative nucleus pulposus (NP) cell. However, the underlying molecular mechanism remains unclear. We here demonstrate that IL-1β played a significan...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5264394/ https://www.ncbi.nlm.nih.gov/pubmed/28120948 http://dx.doi.org/10.1038/srep41067 |
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author | Shen, Jieliang Xu, Shengxi Zhou, Hao Liu, Huzhe Jiang, Wei Hao, Jie Hu, Zhenming |
author_facet | Shen, Jieliang Xu, Shengxi Zhou, Hao Liu, Huzhe Jiang, Wei Hao, Jie Hu, Zhenming |
author_sort | Shen, Jieliang |
collection | PubMed |
description | IL-1β has been reported highly expressed in degenerative intervertebral disc, and our previous study indicated IL-1β facilitates apoptosis of human degenerative nucleus pulposus (NP) cell. However, the underlying molecular mechanism remains unclear. We here demonstrate that IL-1β played a significantly pro-apoptotic effect under serum deprivation. IL-1β decreased Bcl-2/Bax ratio and enhanced cytochrome C released from mitochondria to cytosol, which proved mitochondria-meidated apoptosis was induced. Subsequently, mitochondria damage was detected under IL-1β stimualtion. In addition, IL-1β-mediated injuried mitochondria contributes to activate autophagy. However, pretreatment with the autophagy inhibitor 3-methyladenine showed the potential in further elevating the apoptosis rate induced by IL-1β in NP cells. Our results indicated that the mitochondrial pathway was involved in IL-1β-induced apoptosis of NP cells. Meanwhile, the damaged mitochondria-induced autophagy played a protective role against apoptosis, suggesting a postive feedback mechanism under inflammatory stress. |
format | Online Article Text |
id | pubmed-5264394 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-52643942017-01-30 IL-1β induces apoptosis and autophagy via mitochondria pathway in human degenerative nucleus pulposus cells Shen, Jieliang Xu, Shengxi Zhou, Hao Liu, Huzhe Jiang, Wei Hao, Jie Hu, Zhenming Sci Rep Article IL-1β has been reported highly expressed in degenerative intervertebral disc, and our previous study indicated IL-1β facilitates apoptosis of human degenerative nucleus pulposus (NP) cell. However, the underlying molecular mechanism remains unclear. We here demonstrate that IL-1β played a significantly pro-apoptotic effect under serum deprivation. IL-1β decreased Bcl-2/Bax ratio and enhanced cytochrome C released from mitochondria to cytosol, which proved mitochondria-meidated apoptosis was induced. Subsequently, mitochondria damage was detected under IL-1β stimualtion. In addition, IL-1β-mediated injuried mitochondria contributes to activate autophagy. However, pretreatment with the autophagy inhibitor 3-methyladenine showed the potential in further elevating the apoptosis rate induced by IL-1β in NP cells. Our results indicated that the mitochondrial pathway was involved in IL-1β-induced apoptosis of NP cells. Meanwhile, the damaged mitochondria-induced autophagy played a protective role against apoptosis, suggesting a postive feedback mechanism under inflammatory stress. Nature Publishing Group 2017-01-25 /pmc/articles/PMC5264394/ /pubmed/28120948 http://dx.doi.org/10.1038/srep41067 Text en Copyright © 2017, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Shen, Jieliang Xu, Shengxi Zhou, Hao Liu, Huzhe Jiang, Wei Hao, Jie Hu, Zhenming IL-1β induces apoptosis and autophagy via mitochondria pathway in human degenerative nucleus pulposus cells |
title | IL-1β induces apoptosis and autophagy via mitochondria pathway in human degenerative nucleus pulposus cells |
title_full | IL-1β induces apoptosis and autophagy via mitochondria pathway in human degenerative nucleus pulposus cells |
title_fullStr | IL-1β induces apoptosis and autophagy via mitochondria pathway in human degenerative nucleus pulposus cells |
title_full_unstemmed | IL-1β induces apoptosis and autophagy via mitochondria pathway in human degenerative nucleus pulposus cells |
title_short | IL-1β induces apoptosis and autophagy via mitochondria pathway in human degenerative nucleus pulposus cells |
title_sort | il-1β induces apoptosis and autophagy via mitochondria pathway in human degenerative nucleus pulposus cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5264394/ https://www.ncbi.nlm.nih.gov/pubmed/28120948 http://dx.doi.org/10.1038/srep41067 |
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