A simple and efficient method for poly-3-hydroxybutyrate quantification in diazotrophic bacteria within 5 minutes using flow cytometry

The conventional method for quantification of polyhydroxyalkanoates based on whole-cell methanolysis and gas chromatography (GC) is laborious and time-consuming. In this work, a method based on flow cytometry of Nile red stained bacterial cells was established to quantify poly-3-hydroxybutyrate (PHB...

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Detalles Bibliográficos
Autores principales: Alves, L.P.S., Almeida, A.T., Cruz, L.M., Pedrosa, F.O., de Souza, E.M., Chubatsu, L.S., Müller-Santos, M., Valdameri, G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Associação Brasileira de Divulgação Científica 2017
Materias:
Biomedical Sciences
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5264536/
https://www.ncbi.nlm.nih.gov/pubmed/28099582
http://dx.doi.org/10.1590/1414-431X20165492

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5264536/
https://www.ncbi.nlm.nih.gov/pubmed/28099582
http://dx.doi.org/10.1590/1414-431X20165492

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