Cargando…
A simple and efficient method for poly-3-hydroxybutyrate quantification in diazotrophic bacteria within 5 minutes using flow cytometry
The conventional method for quantification of polyhydroxyalkanoates based on whole-cell methanolysis and gas chromatography (GC) is laborious and time-consuming. In this work, a method based on flow cytometry of Nile red stained bacterial cells was established to quantify poly-3-hydroxybutyrate (PHB...
Autores principales: | Alves, L.P.S., Almeida, A.T., Cruz, L.M., Pedrosa, F.O., de Souza, E.M., Chubatsu, L.S., Müller-Santos, M., Valdameri, G. |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Associação Brasileira de Divulgação Científica
2017
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5264536/ https://www.ncbi.nlm.nih.gov/pubmed/28099582 http://dx.doi.org/10.1590/1414-431X20165492 |
Ejemplares similares
-
Effect of point mutations on Herbaspirillum seropedicae
NifA activity
por: Aquino, B., et al.
Publicado: (2015) -
A broad pH range and processive chitinase from a metagenome
library
por: Thimoteo, S.S., et al.
Publicado: (2017) -
Effect of ATP and 2-oxoglutarate on the in vitro interaction between the NifA GAF domain and the GlnB protein of Azospirillum brasilense
por: Sotomaior, P., et al.
Publicado: (2012) -
Expression and characterization of an N-truncated form of the NifA protein of Azospirillum brasilense
por: Nishikawa, C.Y., et al.
Publicado: (2012) -
Trypan blue exclusion assay by flow
cytometry
por: Avelar-Freitas, B.A., et al.
Publicado: (2014)