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A moderate increase in dietary zinc reduces DNA strand breaks in leukocytes and alters plasma proteins without changing plasma zinc concentrations(1)(2)(3)

Background: Food fortification has been recommended to improve a population’s micronutrient status. Biofortification techniques modestly elevate the zinc content of cereals, but few studies have reported a positive impact on functional indicators of zinc status. Objective: We determined the impact o...

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Autores principales: Zyba, Sarah J, Shenvi, Swapna V, Killilea, David W, Holland, Tai C, Kim, Elijah, Moy, Adrian, Sutherland, Barbara, Gildengorin, Virginia, Shigenaga, Mark K, King, Janet C
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Nutrition 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5267297/
https://www.ncbi.nlm.nih.gov/pubmed/28003206
http://dx.doi.org/10.3945/ajcn.116.135327
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author Zyba, Sarah J
Shenvi, Swapna V
Killilea, David W
Holland, Tai C
Kim, Elijah
Moy, Adrian
Sutherland, Barbara
Gildengorin, Virginia
Shigenaga, Mark K
King, Janet C
author_facet Zyba, Sarah J
Shenvi, Swapna V
Killilea, David W
Holland, Tai C
Kim, Elijah
Moy, Adrian
Sutherland, Barbara
Gildengorin, Virginia
Shigenaga, Mark K
King, Janet C
author_sort Zyba, Sarah J
collection PubMed
description Background: Food fortification has been recommended to improve a population’s micronutrient status. Biofortification techniques modestly elevate the zinc content of cereals, but few studies have reported a positive impact on functional indicators of zinc status. Objective: We determined the impact of a modest increase in dietary zinc that was similar to that provided by biofortification programs on whole-body and cellular indicators of zinc status. Design: Eighteen men participated in a 6-wk controlled consumption study of a low-zinc, rice-based diet. The diet contained 6 mg Zn/d for 2 wk and was followed by 10 mg Zn/d for 4 wk. To reduce zinc absorption, phytate was added to the diet during the initial period. Indicators of zinc homeostasis, including total absorbed zinc (TAZ), the exchangeable zinc pool (EZP), plasma and cellular zinc concentrations, zinc transporter gene expression, and other metabolic indicators (i.e., DNA damage, inflammation, and oxidative stress), were measured before and after each dietary-zinc period. Results: TAZ increased with increased dietary zinc, but plasma zinc concentrations and EZP size were unchanged. Erythrocyte and leukocyte zinc concentrations and zinc transporter expressions were not altered. However, leukocyte DNA strand breaks decreased with increased dietary zinc, and the level of proteins involved in DNA repair and antioxidant and immune functions were restored after the dietary-zinc increase. Conclusions: A moderate 4-mg/d increase in dietary zinc, similar to that which would be expected from zinc-biofortified crops, improves zinc absorption but does not alter plasma zinc. The repair of DNA strand breaks improves, as do serum protein concentrations that are associated with the DNA repair process. This trial was registered at clinicaltrials.gov as NCT02861352.
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spelling pubmed-52672972017-09-12 A moderate increase in dietary zinc reduces DNA strand breaks in leukocytes and alters plasma proteins without changing plasma zinc concentrations(1)(2)(3) Zyba, Sarah J Shenvi, Swapna V Killilea, David W Holland, Tai C Kim, Elijah Moy, Adrian Sutherland, Barbara Gildengorin, Virginia Shigenaga, Mark K King, Janet C Am J Clin Nutr Vitamins, Minerals, and Phytochemicals Background: Food fortification has been recommended to improve a population’s micronutrient status. Biofortification techniques modestly elevate the zinc content of cereals, but few studies have reported a positive impact on functional indicators of zinc status. Objective: We determined the impact of a modest increase in dietary zinc that was similar to that provided by biofortification programs on whole-body and cellular indicators of zinc status. Design: Eighteen men participated in a 6-wk controlled consumption study of a low-zinc, rice-based diet. The diet contained 6 mg Zn/d for 2 wk and was followed by 10 mg Zn/d for 4 wk. To reduce zinc absorption, phytate was added to the diet during the initial period. Indicators of zinc homeostasis, including total absorbed zinc (TAZ), the exchangeable zinc pool (EZP), plasma and cellular zinc concentrations, zinc transporter gene expression, and other metabolic indicators (i.e., DNA damage, inflammation, and oxidative stress), were measured before and after each dietary-zinc period. Results: TAZ increased with increased dietary zinc, but plasma zinc concentrations and EZP size were unchanged. Erythrocyte and leukocyte zinc concentrations and zinc transporter expressions were not altered. However, leukocyte DNA strand breaks decreased with increased dietary zinc, and the level of proteins involved in DNA repair and antioxidant and immune functions were restored after the dietary-zinc increase. Conclusions: A moderate 4-mg/d increase in dietary zinc, similar to that which would be expected from zinc-biofortified crops, improves zinc absorption but does not alter plasma zinc. The repair of DNA strand breaks improves, as do serum protein concentrations that are associated with the DNA repair process. This trial was registered at clinicaltrials.gov as NCT02861352. American Society for Nutrition 2017-02 2016-12-21 /pmc/articles/PMC5267297/ /pubmed/28003206 http://dx.doi.org/10.3945/ajcn.116.135327 Text en http://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the CC-BY license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Vitamins, Minerals, and Phytochemicals
Zyba, Sarah J
Shenvi, Swapna V
Killilea, David W
Holland, Tai C
Kim, Elijah
Moy, Adrian
Sutherland, Barbara
Gildengorin, Virginia
Shigenaga, Mark K
King, Janet C
A moderate increase in dietary zinc reduces DNA strand breaks in leukocytes and alters plasma proteins without changing plasma zinc concentrations(1)(2)(3)
title A moderate increase in dietary zinc reduces DNA strand breaks in leukocytes and alters plasma proteins without changing plasma zinc concentrations(1)(2)(3)
title_full A moderate increase in dietary zinc reduces DNA strand breaks in leukocytes and alters plasma proteins without changing plasma zinc concentrations(1)(2)(3)
title_fullStr A moderate increase in dietary zinc reduces DNA strand breaks in leukocytes and alters plasma proteins without changing plasma zinc concentrations(1)(2)(3)
title_full_unstemmed A moderate increase in dietary zinc reduces DNA strand breaks in leukocytes and alters plasma proteins without changing plasma zinc concentrations(1)(2)(3)
title_short A moderate increase in dietary zinc reduces DNA strand breaks in leukocytes and alters plasma proteins without changing plasma zinc concentrations(1)(2)(3)
title_sort moderate increase in dietary zinc reduces dna strand breaks in leukocytes and alters plasma proteins without changing plasma zinc concentrations(1)(2)(3)
topic Vitamins, Minerals, and Phytochemicals
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5267297/
https://www.ncbi.nlm.nih.gov/pubmed/28003206
http://dx.doi.org/10.3945/ajcn.116.135327
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