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Differences in AMY1 Gene Copy Numbers Derived from Blood, Buccal Cells and Saliva Using Quantitative and Droplet Digital PCR Methods: Flagging the Pitfall

INTRODUCTION: The human salivary (AMY1) gene, encoding salivary α-amylase, has variable copy number variants (CNVs) in the human genome. We aimed to determine if real-time quantitative polymerase chain reaction (qPCR) and the more recently available Droplet Digital PCR (ddPCR) can provide a precise...

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Detalles Bibliográficos
Autores principales: Ooi, Delicia Shu Qin, Tan, Verena Ming Hui, Ong, Siong Gim, Chan, Yiong Huak, Heng, Chew Kiat, Lee, Yung Seng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5268653/
https://www.ncbi.nlm.nih.gov/pubmed/28125683
http://dx.doi.org/10.1371/journal.pone.0170767