Aging promotes neoplastic disease through effects on the tissue microenvironment
A better understanding of the complex relationship between aging and cancer will provide important tools for the prevention and treatment of neoplasia. In these studies, the hypothesis was tested that aging may fuel carcinogenesis via alterations imposed in the tissue microenvironment. Preneoplastic...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Impact Journals LLC
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5270675/ https://www.ncbi.nlm.nih.gov/pubmed/27929382 http://dx.doi.org/10.18632/aging.101128 |
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author | Marongiu, Fabio Paola Serra, Maria Doratiotto, Silvia Sini, Marcella Fanti, Maura Cadoni, Erika Serra, Monica Laconi, Ezio |
author_facet | Marongiu, Fabio Paola Serra, Maria Doratiotto, Silvia Sini, Marcella Fanti, Maura Cadoni, Erika Serra, Monica Laconi, Ezio |
author_sort | Marongiu, Fabio |
collection | PubMed |
description | A better understanding of the complex relationship between aging and cancer will provide important tools for the prevention and treatment of neoplasia. In these studies, the hypothesis was tested that aging may fuel carcinogenesis via alterations imposed in the tissue microenvironment. Preneoplastic hepatocytes isolated from liver nodules were orthotopically injected into either young or old syngeneic rats and their fate was followed over time using the dipeptidyl-peptidase type IV (DPPIV) system to track donor-derived-cells. At 3 months post-Tx, the mean size of donor-derived clusters was 11±3 cells in young vs. 42±8 in old recipients. At 8 months post-Tx, no visible lesion were detected in any of 21 young recipients, while 17/18 animals transplanted at old age displayed hepatic nodules, including 7 large tumors. All tumors expressed the DPPIV marker enzyme, indicating that they originated from transplanted cells. Expression of senescence-associated β-galactosidase was common in liver of 18-month old animals, while it was a rare finding in young controls. Finally, both mRNA and IL6 protein were found to be increased in the liver of aged rats compared to young controls. These results are interpreted to indicate that the microenvironment of the aged liver promotes the growth of pre-neoplastic hepatocytes. |
format | Online Article Text |
id | pubmed-5270675 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Impact Journals LLC |
record_format | MEDLINE/PubMed |
spelling | pubmed-52706752017-01-27 Aging promotes neoplastic disease through effects on the tissue microenvironment Marongiu, Fabio Paola Serra, Maria Doratiotto, Silvia Sini, Marcella Fanti, Maura Cadoni, Erika Serra, Monica Laconi, Ezio Aging (Albany NY) Research Paper A better understanding of the complex relationship between aging and cancer will provide important tools for the prevention and treatment of neoplasia. In these studies, the hypothesis was tested that aging may fuel carcinogenesis via alterations imposed in the tissue microenvironment. Preneoplastic hepatocytes isolated from liver nodules were orthotopically injected into either young or old syngeneic rats and their fate was followed over time using the dipeptidyl-peptidase type IV (DPPIV) system to track donor-derived-cells. At 3 months post-Tx, the mean size of donor-derived clusters was 11±3 cells in young vs. 42±8 in old recipients. At 8 months post-Tx, no visible lesion were detected in any of 21 young recipients, while 17/18 animals transplanted at old age displayed hepatic nodules, including 7 large tumors. All tumors expressed the DPPIV marker enzyme, indicating that they originated from transplanted cells. Expression of senescence-associated β-galactosidase was common in liver of 18-month old animals, while it was a rare finding in young controls. Finally, both mRNA and IL6 protein were found to be increased in the liver of aged rats compared to young controls. These results are interpreted to indicate that the microenvironment of the aged liver promotes the growth of pre-neoplastic hepatocytes. Impact Journals LLC 2016-12-06 /pmc/articles/PMC5270675/ /pubmed/27929382 http://dx.doi.org/10.18632/aging.101128 Text en Copyright: © 2016 Marongiu et al. http://creativecommons.org/licenses/by/3.0/ This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/) (CC-BY), which permits unrestricted use and redistribution provided that the original author and source are credited. |
spellingShingle | Research Paper Marongiu, Fabio Paola Serra, Maria Doratiotto, Silvia Sini, Marcella Fanti, Maura Cadoni, Erika Serra, Monica Laconi, Ezio Aging promotes neoplastic disease through effects on the tissue microenvironment |
title | Aging promotes neoplastic disease through effects on the tissue microenvironment |
title_full | Aging promotes neoplastic disease through effects on the tissue microenvironment |
title_fullStr | Aging promotes neoplastic disease through effects on the tissue microenvironment |
title_full_unstemmed | Aging promotes neoplastic disease through effects on the tissue microenvironment |
title_short | Aging promotes neoplastic disease through effects on the tissue microenvironment |
title_sort | aging promotes neoplastic disease through effects on the tissue microenvironment |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5270675/ https://www.ncbi.nlm.nih.gov/pubmed/27929382 http://dx.doi.org/10.18632/aging.101128 |
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