Combining multi-mutant and modular thermodynamic cycles to measure energetic coupling networks in enzyme catalysis

We measured and cross-validated the energetics of networks in Bacillus stearothermophilus Tryptophanyl-tRNA synthetase (TrpRS) using both multi-mutant and modular thermodynamic cycles. Multi-dimensional combinatorial mutagenesis showed that four side chains from this “molecular switch” move coordina...

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Detalles Bibliográficos
Autores principales: Carter, Charles W., Chandrasekaran, Srinivas Niranj, Weinreb, Violetta, Li, Li, Williams, Tishan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Crystallographic Association 2017
Materias:
Transactions from the 66th Annual Meeting of the American Crystallographic Association (Aca)
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5272822/
https://www.ncbi.nlm.nih.gov/pubmed/28191480
http://dx.doi.org/10.1063/1.4974218

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5272822/
https://www.ncbi.nlm.nih.gov/pubmed/28191480
http://dx.doi.org/10.1063/1.4974218

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