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A new model of sperm nuclear architecture following assessment of the organization of centromeres and telomeres in three-dimensions

The organization of chromosomes in sperm nuclei has been proposed to possess a unique “hairpin-loop” arrangement, which is hypothesized to aid in the ordered exodus of the paternal genome following fertilization. This study simultaneously assessed the 3D and 2D radial and longitudinal organization o...

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Autores principales: Ioannou, Dimitrios, Millan, Nicole M., Jordan, Elizabeth, Tempest, Helen G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5282497/
https://www.ncbi.nlm.nih.gov/pubmed/28139771
http://dx.doi.org/10.1038/srep41585
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author Ioannou, Dimitrios
Millan, Nicole M.
Jordan, Elizabeth
Tempest, Helen G.
author_facet Ioannou, Dimitrios
Millan, Nicole M.
Jordan, Elizabeth
Tempest, Helen G.
author_sort Ioannou, Dimitrios
collection PubMed
description The organization of chromosomes in sperm nuclei has been proposed to possess a unique “hairpin-loop” arrangement, which is hypothesized to aid in the ordered exodus of the paternal genome following fertilization. This study simultaneously assessed the 3D and 2D radial and longitudinal organization of telomeres, centromeres, and investigated whether chromosomes formed the same centromere clusters in sperm cells. Reproducible radial and longitudinal non-random organization was observed for all investigated loci using both 3D and 2D approaches in multiple subjects. We report novel findings, with telomeres and centromeres being localized throughout the nucleus but demonstrating roughly a 1:1 distribution in the nuclear periphery and the intermediate regions with <15% occupying the nuclear interior. Telomeres and centromeres were observed to aggregate in sperm nuclei, forming an average of 20 and 7 clusters, respectively. Reproducible longitudinal organization demonstrated preferential localization of telomeres and centromeres in the mid region of the sperm cell. Preliminary evidence is also provided to support the hypothesis that specific chromosomes preferentially form the same centromere clusters. The more segmental distribution of telomeres and centromeres as described in this study could more readily accommodate and facilitate the sequential exodus of paternal chromosomes following fertilization.
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spelling pubmed-52824972017-02-03 A new model of sperm nuclear architecture following assessment of the organization of centromeres and telomeres in three-dimensions Ioannou, Dimitrios Millan, Nicole M. Jordan, Elizabeth Tempest, Helen G. Sci Rep Article The organization of chromosomes in sperm nuclei has been proposed to possess a unique “hairpin-loop” arrangement, which is hypothesized to aid in the ordered exodus of the paternal genome following fertilization. This study simultaneously assessed the 3D and 2D radial and longitudinal organization of telomeres, centromeres, and investigated whether chromosomes formed the same centromere clusters in sperm cells. Reproducible radial and longitudinal non-random organization was observed for all investigated loci using both 3D and 2D approaches in multiple subjects. We report novel findings, with telomeres and centromeres being localized throughout the nucleus but demonstrating roughly a 1:1 distribution in the nuclear periphery and the intermediate regions with <15% occupying the nuclear interior. Telomeres and centromeres were observed to aggregate in sperm nuclei, forming an average of 20 and 7 clusters, respectively. Reproducible longitudinal organization demonstrated preferential localization of telomeres and centromeres in the mid region of the sperm cell. Preliminary evidence is also provided to support the hypothesis that specific chromosomes preferentially form the same centromere clusters. The more segmental distribution of telomeres and centromeres as described in this study could more readily accommodate and facilitate the sequential exodus of paternal chromosomes following fertilization. Nature Publishing Group 2017-01-31 /pmc/articles/PMC5282497/ /pubmed/28139771 http://dx.doi.org/10.1038/srep41585 Text en Copyright © 2017, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Ioannou, Dimitrios
Millan, Nicole M.
Jordan, Elizabeth
Tempest, Helen G.
A new model of sperm nuclear architecture following assessment of the organization of centromeres and telomeres in three-dimensions
title A new model of sperm nuclear architecture following assessment of the organization of centromeres and telomeres in three-dimensions
title_full A new model of sperm nuclear architecture following assessment of the organization of centromeres and telomeres in three-dimensions
title_fullStr A new model of sperm nuclear architecture following assessment of the organization of centromeres and telomeres in three-dimensions
title_full_unstemmed A new model of sperm nuclear architecture following assessment of the organization of centromeres and telomeres in three-dimensions
title_short A new model of sperm nuclear architecture following assessment of the organization of centromeres and telomeres in three-dimensions
title_sort new model of sperm nuclear architecture following assessment of the organization of centromeres and telomeres in three-dimensions
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5282497/
https://www.ncbi.nlm.nih.gov/pubmed/28139771
http://dx.doi.org/10.1038/srep41585
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