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Droplet-based microfluidic high-throughput screening of heterologous enzymes secreted by the yeast Yarrowia lipolytica

BACKGROUND: Droplet-based microfluidics is becoming an increasingly attractive alternative to microtiter plate techniques for enzymatic high-throughput screening (HTS), especially for exploring large diversities with lower time and cost footprint. In this case, the assayed enzyme has to be accessibl...

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Detalles Bibliográficos
Autores principales: Beneyton, Thomas, Thomas, Stéphane, Griffiths, Andrew D., Nicaud, Jean-Marc, Drevelle, Antoine, Rossignol, Tristan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5282883/
https://www.ncbi.nlm.nih.gov/pubmed/28143479
http://dx.doi.org/10.1186/s12934-017-0629-5