The human initiator is a distinct and abundant element that is precisely positioned in focused core promoters

DNA sequence signals in the core promoter, such as the initiator (Inr), direct transcription initiation by RNA polymerase II. Here we show that the human Inr has the consensus of BBCA(+1)BW at focused promoters in which transcription initiates at a single site or a narrow cluster of sites. The analy...

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Detalles Bibliográficos
Autores principales: Vo ngoc, Long, Cassidy, California Jack, Huang, Cassidy Yunjing, Duttke, Sascha H.C., Kadonaga, James T.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory Press 2017
Materias:
Research Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5287114/
https://www.ncbi.nlm.nih.gov/pubmed/28108474
http://dx.doi.org/10.1101/gad.293837.116
Descripción
Sumario:DNA sequence signals in the core promoter, such as the initiator (Inr), direct transcription initiation by RNA polymerase II. Here we show that the human Inr has the consensus of BBCA(+1)BW at focused promoters in which transcription initiates at a single site or a narrow cluster of sites. The analysis of 7678 focused transcription start sites revealed 40% with a perfect match to the Inr and 16% with a single mismatch outside of the CA(+1) core. TATA-like sequences are underrepresented in Inr promoters. This consensus is a key component of the DNA sequence rules that specify transcription initiation in humans.

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