The human initiator is a distinct and abundant element that is precisely positioned in focused core promoters

DNA sequence signals in the core promoter, such as the initiator (Inr), direct transcription initiation by RNA polymerase II. Here we show that the human Inr has the consensus of BBCA(+1)BW at focused promoters in which transcription initiates at a single site or a narrow cluster of sites. The analy...

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Detalles Bibliográficos
Autores principales: Vo ngoc, Long, Cassidy, California Jack, Huang, Cassidy Yunjing, Duttke, Sascha H.C., Kadonaga, James T.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory Press 2017
Materias:
Research Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5287114/
https://www.ncbi.nlm.nih.gov/pubmed/28108474
http://dx.doi.org/10.1101/gad.293837.116
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author Vo ngoc, Long
Cassidy, California Jack
Huang, Cassidy Yunjing
Duttke, Sascha H.C.
Kadonaga, James T.
author_facet Vo ngoc, Long
Cassidy, California Jack
Huang, Cassidy Yunjing
Duttke, Sascha H.C.
Kadonaga, James T.
author_sort Vo ngoc, Long
collection PubMed
description DNA sequence signals in the core promoter, such as the initiator (Inr), direct transcription initiation by RNA polymerase II. Here we show that the human Inr has the consensus of BBCA(+1)BW at focused promoters in which transcription initiates at a single site or a narrow cluster of sites. The analysis of 7678 focused transcription start sites revealed 40% with a perfect match to the Inr and 16% with a single mismatch outside of the CA(+1) core. TATA-like sequences are underrepresented in Inr promoters. This consensus is a key component of the DNA sequence rules that specify transcription initiation in humans.
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spelling pubmed-52871142017-07-01 The human initiator is a distinct and abundant element that is precisely positioned in focused core promoters Vo ngoc, Long Cassidy, California Jack Huang, Cassidy Yunjing Duttke, Sascha H.C. Kadonaga, James T. Genes Dev Research Communication DNA sequence signals in the core promoter, such as the initiator (Inr), direct transcription initiation by RNA polymerase II. Here we show that the human Inr has the consensus of BBCA(+1)BW at focused promoters in which transcription initiates at a single site or a narrow cluster of sites. The analysis of 7678 focused transcription start sites revealed 40% with a perfect match to the Inr and 16% with a single mismatch outside of the CA(+1) core. TATA-like sequences are underrepresented in Inr promoters. This consensus is a key component of the DNA sequence rules that specify transcription initiation in humans. Cold Spring Harbor Laboratory Press 2017-01-01 /pmc/articles/PMC5287114/ /pubmed/28108474 http://dx.doi.org/10.1101/gad.293837.116 Text en © 2017 Vo ngoc et al.; Published by Cold Spring Harbor Laboratory Press http://creativecommons.org/licenses/by-nc/4.0/ This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genesdev.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.
spellingShingle Research Communication
Vo ngoc, Long
Cassidy, California Jack
Huang, Cassidy Yunjing
Duttke, Sascha H.C.
Kadonaga, James T.
The human initiator is a distinct and abundant element that is precisely positioned in focused core promoters
title The human initiator is a distinct and abundant element that is precisely positioned in focused core promoters
title_full The human initiator is a distinct and abundant element that is precisely positioned in focused core promoters
title_fullStr The human initiator is a distinct and abundant element that is precisely positioned in focused core promoters
title_full_unstemmed The human initiator is a distinct and abundant element that is precisely positioned in focused core promoters
title_short The human initiator is a distinct and abundant element that is precisely positioned in focused core promoters
title_sort human initiator is a distinct and abundant element that is precisely positioned in focused core promoters
topic Research Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5287114/
https://www.ncbi.nlm.nih.gov/pubmed/28108474
http://dx.doi.org/10.1101/gad.293837.116
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