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APOBEC3A/B-induced mutagenesis is responsible for 20% of heritable mutations in the TpCpW context

APOBEC3A/B cytidine deaminase is responsible for the majority of cancerous mutations in a large fraction of cancer samples. However, its role in heritable mutagenesis remains very poorly understood. Recent studies have demonstrated that both in yeast and in human cancerous cells, most APOBEC3A/B-ind...

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Autores principales: Seplyarskiy, Vladimir B., Andrianova, Maria A., Bazykin, Georgii A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory Press 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5287224/
https://www.ncbi.nlm.nih.gov/pubmed/27940951
http://dx.doi.org/10.1101/gr.210336.116
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author Seplyarskiy, Vladimir B.
Andrianova, Maria A.
Bazykin, Georgii A.
author_facet Seplyarskiy, Vladimir B.
Andrianova, Maria A.
Bazykin, Georgii A.
author_sort Seplyarskiy, Vladimir B.
collection PubMed
description APOBEC3A/B cytidine deaminase is responsible for the majority of cancerous mutations in a large fraction of cancer samples. However, its role in heritable mutagenesis remains very poorly understood. Recent studies have demonstrated that both in yeast and in human cancerous cells, most APOBEC3A/B-induced mutations occur on the lagging strand during replication and on the nontemplate strand of transcribed regions. Here, we use data on rare human polymorphisms, interspecies divergence, and de novo mutations to study germline mutagenesis and to analyze mutations at nucleotide contexts prone to attack by APOBEC3A/B. We show that such mutations occur preferentially on the lagging strand and on nontemplate strands of transcribed regions. Moreover, we demonstrate that APOBEC3A/B-like mutations tend to produce strand-coordinated clusters, which are also biased toward the lagging strand. Finally, we show that the mutation rate is increased 3′ of C→G mutations to a greater extent than 3′ of C→T mutations, suggesting pervasive trans-lesion bypass of the APOBEC3A/B-induced damage. Our study demonstrates that 20% of C→T and C→G mutations in the TpCpW context—where W denotes A or T, segregating as polymorphisms in human population—or 1.4% of all heritable mutations are attributable to APOBEC3A/B activity.
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spelling pubmed-52872242017-08-01 APOBEC3A/B-induced mutagenesis is responsible for 20% of heritable mutations in the TpCpW context Seplyarskiy, Vladimir B. Andrianova, Maria A. Bazykin, Georgii A. Genome Res Research APOBEC3A/B cytidine deaminase is responsible for the majority of cancerous mutations in a large fraction of cancer samples. However, its role in heritable mutagenesis remains very poorly understood. Recent studies have demonstrated that both in yeast and in human cancerous cells, most APOBEC3A/B-induced mutations occur on the lagging strand during replication and on the nontemplate strand of transcribed regions. Here, we use data on rare human polymorphisms, interspecies divergence, and de novo mutations to study germline mutagenesis and to analyze mutations at nucleotide contexts prone to attack by APOBEC3A/B. We show that such mutations occur preferentially on the lagging strand and on nontemplate strands of transcribed regions. Moreover, we demonstrate that APOBEC3A/B-like mutations tend to produce strand-coordinated clusters, which are also biased toward the lagging strand. Finally, we show that the mutation rate is increased 3′ of C→G mutations to a greater extent than 3′ of C→T mutations, suggesting pervasive trans-lesion bypass of the APOBEC3A/B-induced damage. Our study demonstrates that 20% of C→T and C→G mutations in the TpCpW context—where W denotes A or T, segregating as polymorphisms in human population—or 1.4% of all heritable mutations are attributable to APOBEC3A/B activity. Cold Spring Harbor Laboratory Press 2017-02 /pmc/articles/PMC5287224/ /pubmed/27940951 http://dx.doi.org/10.1101/gr.210336.116 Text en © 2017 Seplyarskiy et al.; Published by Cold Spring Harbor Laboratory Press http://creativecommons.org/licenses/by-nc/4.0/ This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genome.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.
spellingShingle Research
Seplyarskiy, Vladimir B.
Andrianova, Maria A.
Bazykin, Georgii A.
APOBEC3A/B-induced mutagenesis is responsible for 20% of heritable mutations in the TpCpW context
title APOBEC3A/B-induced mutagenesis is responsible for 20% of heritable mutations in the TpCpW context
title_full APOBEC3A/B-induced mutagenesis is responsible for 20% of heritable mutations in the TpCpW context
title_fullStr APOBEC3A/B-induced mutagenesis is responsible for 20% of heritable mutations in the TpCpW context
title_full_unstemmed APOBEC3A/B-induced mutagenesis is responsible for 20% of heritable mutations in the TpCpW context
title_short APOBEC3A/B-induced mutagenesis is responsible for 20% of heritable mutations in the TpCpW context
title_sort apobec3a/b-induced mutagenesis is responsible for 20% of heritable mutations in the tpcpw context
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5287224/
https://www.ncbi.nlm.nih.gov/pubmed/27940951
http://dx.doi.org/10.1101/gr.210336.116
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