3,4-Dihydroxyphenylacetate 2,3-dioxygenase from Pseudomonas aeruginosa: An Fe(II)-containing enzyme with fast turnover

3,4-dihydroxyphenylacetate (DHPA) dioxygenase (DHPAO) from Pseudomonas aeruginosa (PaDHPAO) was overexpressed in Escherichia coli and purified to homogeneity. As the enzyme lost activity over time, a protocol to reactivate and conserve PaDHPAO activity has been developed. Addition of Fe(II), DTT and...

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Detalles Bibliográficos
Autores principales: Pornsuwan, Soraya, Maenpuen, Somchart, Kamutira, Philaiwarong, Watthaisong, Pratchaya, Thotsaporn, Kittisak, Tongsook, Chanakan, Juttulapa, Maneerat, Nijvipakul, Sarayut, Chaiyen, Pimchai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5291488/
https://www.ncbi.nlm.nih.gov/pubmed/28158217
http://dx.doi.org/10.1371/journal.pone.0171135

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5291488/
https://www.ncbi.nlm.nih.gov/pubmed/28158217
http://dx.doi.org/10.1371/journal.pone.0171135

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