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3,4-Dihydroxyphenylacetate 2,3-dioxygenase from Pseudomonas aeruginosa: An Fe(II)-containing enzyme with fast turnover
3,4-dihydroxyphenylacetate (DHPA) dioxygenase (DHPAO) from Pseudomonas aeruginosa (PaDHPAO) was overexpressed in Escherichia coli and purified to homogeneity. As the enzyme lost activity over time, a protocol to reactivate and conserve PaDHPAO activity has been developed. Addition of Fe(II), DTT and...
Autores principales: | Pornsuwan, Soraya, Maenpuen, Somchart, Kamutira, Philaiwarong, Watthaisong, Pratchaya, Thotsaporn, Kittisak, Tongsook, Chanakan, Juttulapa, Maneerat, Nijvipakul, Sarayut, Chaiyen, Pimchai |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5291488/ https://www.ncbi.nlm.nih.gov/pubmed/28158217 http://dx.doi.org/10.1371/journal.pone.0171135 |
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