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High-throughput sequencing of multiple amplicons for barcoding and integrative taxonomy

Until now, the potential of NGS for the construction of barcode libraries or integrative taxonomy has been seldom realised. Here, we amplified (two-step PCR) and simultaneously sequenced (MiSeq) multiple markers from hundreds of fig wasp specimens. We also developed a workflow for quality control of...

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Detalles Bibliográficos
Autores principales: Cruaud, Perrine, Rasplus, Jean-Yves, Rodriguez, Lillian Jennifer, Cruaud, Astrid
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5292727/
https://www.ncbi.nlm.nih.gov/pubmed/28165046
http://dx.doi.org/10.1038/srep41948