Unraveling CRISPR-Cas9 genome engineering parameters via a library-on-library approach

We develop an in vivo library-on-library methodology to simultaneously assess single guide RNA (sgRNA) activity across ~1,400 genomic loci. Assaying across multiple human cell types, end-processing enzymes, and two Cas9 orthologs, we unravel underlying nucleotide sequence and epigenetic parameters....

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Detalles Bibliográficos
Autores principales: Chari, Raj, Mali, Prashant, Moosburner, Mark, Church, George M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2015
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5292764/
https://www.ncbi.nlm.nih.gov/pubmed/26167643
http://dx.doi.org/10.1038/nmeth.3473

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5292764/
https://www.ncbi.nlm.nih.gov/pubmed/26167643
http://dx.doi.org/10.1038/nmeth.3473

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