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Unraveling CRISPR-Cas9 genome engineering parameters via a library-on-library approach

We develop an in vivo library-on-library methodology to simultaneously assess single guide RNA (sgRNA) activity across ~1,400 genomic loci. Assaying across multiple human cell types, end-processing enzymes, and two Cas9 orthologs, we unravel underlying nucleotide sequence and epigenetic parameters....

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Detalles Bibliográficos
Autores principales:	Chari, Raj, Mali, Prashant, Moosburner, Mark, Church, George M.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	2015
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5292764/ https://www.ncbi.nlm.nih.gov/pubmed/26167643 http://dx.doi.org/10.1038/nmeth.3473

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