Engineering a trifunctional proline utilization A chimaera by fusing a DNA-binding domain to a bifunctional PutA

Proline utilization A (PutA) is a bifunctional flavoenzyme with proline dehydrogenase (PRODH) and Δ(1)-pyrroline-5-carboxylate (P5C) dehydrogenase (P5CDH) domains that catalyses the two-step oxidation of proline to glutamate. Trifunctional PutAs also have an N-terminal ribbon–helix–helix (RHH) DNA-b...

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Detalles Bibliográficos
Autores principales: Arentson, Benjamin W., Hayes, Erin L., Zhu, Weidong, Singh, Harkewal, Tanner, John J., Becker, Donald F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Portland Press Ltd. 2016
Materias:
Original Papers
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5293562/
https://www.ncbi.nlm.nih.gov/pubmed/27742866
http://dx.doi.org/10.1042/BSR20160435

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5293562/
https://www.ncbi.nlm.nih.gov/pubmed/27742866
http://dx.doi.org/10.1042/BSR20160435

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