Highly Efficient Cpf1-Mediated Gene Targeting in Mice Following High Concentration Pronuclear Injection

Cpf1 has emerged as an alternative to the Cas9 RNA-guided nuclease. Here we show that gene targeting rates in mice using Cpf1 can meet, or even surpass, Cas9 targeting rates (approaching 100% targeting), but require higher concentrations of mRNA and guide. We also demonstrate that coinjecting two gu...

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Detalles Bibliográficos
Autores principales: Watkins-Chow, Dawn E., Varshney, Gaurav K., Garrett, Lisa J., Chen, Zelin, Jimenez, Erin A., Rivas, Cecilia, Bishop, Kevin S., Sood, Raman, Harper, Ursula L., Pavan, William J., Burgess, Shawn M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Genetics Society of America 2016
Materias:
Investigations
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5295614/
https://www.ncbi.nlm.nih.gov/pubmed/28040780
http://dx.doi.org/10.1534/g3.116.038091

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5295614/
https://www.ncbi.nlm.nih.gov/pubmed/28040780
http://dx.doi.org/10.1534/g3.116.038091

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