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Hydrostatic Pressure Regulates MicroRNA Expression Levels in Osteoarthritic Chondrocyte Cultures via the Wnt/β-Catenin Pathway

Mechanical loading and hydrostatic pressure (HP) regulate chondrocytes’ metabolism; however, how mechanical stimulation acts remain unclear. MicroRNAs (miRNAs) play an important role in cartilage homeostasis, mechanotransduction, and in the pathogenesis of osteoarthritis (OA). This study investigate...

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Autores principales: Cheleschi, Sara, De Palma, Anna, Pecorelli, Alessandra, Pascarelli, Nicola Antonio, Valacchi, Giuseppe, Belmonte, Giuseppe, Carta, Serafino, Galeazzi, Mauro, Fioravanti, Antonella
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5297766/
https://www.ncbi.nlm.nih.gov/pubmed/28085114
http://dx.doi.org/10.3390/ijms18010133
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author Cheleschi, Sara
De Palma, Anna
Pecorelli, Alessandra
Pascarelli, Nicola Antonio
Valacchi, Giuseppe
Belmonte, Giuseppe
Carta, Serafino
Galeazzi, Mauro
Fioravanti, Antonella
author_facet Cheleschi, Sara
De Palma, Anna
Pecorelli, Alessandra
Pascarelli, Nicola Antonio
Valacchi, Giuseppe
Belmonte, Giuseppe
Carta, Serafino
Galeazzi, Mauro
Fioravanti, Antonella
author_sort Cheleschi, Sara
collection PubMed
description Mechanical loading and hydrostatic pressure (HP) regulate chondrocytes’ metabolism; however, how mechanical stimulation acts remain unclear. MicroRNAs (miRNAs) play an important role in cartilage homeostasis, mechanotransduction, and in the pathogenesis of osteoarthritis (OA). This study investigated the effects of a cyclic HP (1–5 MPa), in both normal and OA human chondrocytes, on the expression of miR-27a/b, miR-140, miR-146a/b, and miR-365, and of their target genes (MMP-13, ADAMTS-5, IGFBP-5, and HDAC-4). Furthermore, we assessed the possible involvement of Wnt/β-catenin pathway in response to HP. Chondrocytes were exposed to HP for 3h and the evaluations were performed immediately after pressurization, and following 12, 24, and 48 h. Total RNA was extracted and used for real-time PCR. β-catenin was detected by Western blotting analysis and immunofluorescence. In OA chondrocytes, HP induced a significant increase (p < 0.01) of the expression levels of miR-27a/b, miR-140, and miR-146a, and a significant reduction (p < 0.01) of miR-365 at all analyzed time points. MMP-13, ADAMTS-5, and HDAC-4 were significantly downregulated following HP, while no significant modification was found for IGFBP-5. β-catenin levels were significantly increased (p < 0.001) in OA chondrocytes at basal conditions and significantly reduced (p < 0.01) by HP. Pressurization did not cause any significant modification in normal cells. In conclusion, in OA chondrocytes, HP restores the expression levels of some miRNAs, downregulates MMP-13, ADAMTS-5, and HDAC-4, and modulates the Wnt/β-catenin pathway activation.
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spelling pubmed-52977662017-02-10 Hydrostatic Pressure Regulates MicroRNA Expression Levels in Osteoarthritic Chondrocyte Cultures via the Wnt/β-Catenin Pathway Cheleschi, Sara De Palma, Anna Pecorelli, Alessandra Pascarelli, Nicola Antonio Valacchi, Giuseppe Belmonte, Giuseppe Carta, Serafino Galeazzi, Mauro Fioravanti, Antonella Int J Mol Sci Article Mechanical loading and hydrostatic pressure (HP) regulate chondrocytes’ metabolism; however, how mechanical stimulation acts remain unclear. MicroRNAs (miRNAs) play an important role in cartilage homeostasis, mechanotransduction, and in the pathogenesis of osteoarthritis (OA). This study investigated the effects of a cyclic HP (1–5 MPa), in both normal and OA human chondrocytes, on the expression of miR-27a/b, miR-140, miR-146a/b, and miR-365, and of their target genes (MMP-13, ADAMTS-5, IGFBP-5, and HDAC-4). Furthermore, we assessed the possible involvement of Wnt/β-catenin pathway in response to HP. Chondrocytes were exposed to HP for 3h and the evaluations were performed immediately after pressurization, and following 12, 24, and 48 h. Total RNA was extracted and used for real-time PCR. β-catenin was detected by Western blotting analysis and immunofluorescence. In OA chondrocytes, HP induced a significant increase (p < 0.01) of the expression levels of miR-27a/b, miR-140, and miR-146a, and a significant reduction (p < 0.01) of miR-365 at all analyzed time points. MMP-13, ADAMTS-5, and HDAC-4 were significantly downregulated following HP, while no significant modification was found for IGFBP-5. β-catenin levels were significantly increased (p < 0.001) in OA chondrocytes at basal conditions and significantly reduced (p < 0.01) by HP. Pressurization did not cause any significant modification in normal cells. In conclusion, in OA chondrocytes, HP restores the expression levels of some miRNAs, downregulates MMP-13, ADAMTS-5, and HDAC-4, and modulates the Wnt/β-catenin pathway activation. MDPI 2017-01-12 /pmc/articles/PMC5297766/ /pubmed/28085114 http://dx.doi.org/10.3390/ijms18010133 Text en © 2017 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Cheleschi, Sara
De Palma, Anna
Pecorelli, Alessandra
Pascarelli, Nicola Antonio
Valacchi, Giuseppe
Belmonte, Giuseppe
Carta, Serafino
Galeazzi, Mauro
Fioravanti, Antonella
Hydrostatic Pressure Regulates MicroRNA Expression Levels in Osteoarthritic Chondrocyte Cultures via the Wnt/β-Catenin Pathway
title Hydrostatic Pressure Regulates MicroRNA Expression Levels in Osteoarthritic Chondrocyte Cultures via the Wnt/β-Catenin Pathway
title_full Hydrostatic Pressure Regulates MicroRNA Expression Levels in Osteoarthritic Chondrocyte Cultures via the Wnt/β-Catenin Pathway
title_fullStr Hydrostatic Pressure Regulates MicroRNA Expression Levels in Osteoarthritic Chondrocyte Cultures via the Wnt/β-Catenin Pathway
title_full_unstemmed Hydrostatic Pressure Regulates MicroRNA Expression Levels in Osteoarthritic Chondrocyte Cultures via the Wnt/β-Catenin Pathway
title_short Hydrostatic Pressure Regulates MicroRNA Expression Levels in Osteoarthritic Chondrocyte Cultures via the Wnt/β-Catenin Pathway
title_sort hydrostatic pressure regulates microrna expression levels in osteoarthritic chondrocyte cultures via the wnt/β-catenin pathway
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5297766/
https://www.ncbi.nlm.nih.gov/pubmed/28085114
http://dx.doi.org/10.3390/ijms18010133
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