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Site‐Specific Immobilization of the Peptidoglycan Synthase PBP1B on a Surface Plasmon Resonance Chip Surface

Surface plasmon resonance (SPR) is one of the most powerful label‐free methods to determine the kinetic parameters of molecular interactions in real time and in a highly sensitive way. Penicillin‐binding proteins (PBPs) are peptidoglycan synthesis enzymes present in most bacteria. Established protoc...

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Detalles Bibliográficos
Autores principales: van't Veer, Inge L., Leloup, Nadia O. L., Egan, Alexander J. F., Janssen, Bert J. C., Martin, Nathaniel I., Vollmer, Waldemar, Breukink, Eefjan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5298014/
https://www.ncbi.nlm.nih.gov/pubmed/27709766
http://dx.doi.org/10.1002/cbic.201600461