Cargando…

Orthogonal Cysteine Protection Enables Homogeneous Multi‐Drug Antibody–Drug Conjugates

A strategy for the preparation of homogeneous antibody–drug conjugates (ADCs) containing multiple payloads has been developed. This approach utilizes sequential unmasking of cysteine residues with orthogonal protection to enable site‐specific conjugation of each drug. In addition, because the approa...

Descripción completa

Detalles Bibliográficos
Autores principales:	Levengood, Matthew R., Zhang, Xinqun, Hunter, Joshua H., Emmerton, Kim K., Miyamoto, Jamie B., Lewis, Timothy S., Senter, Peter D.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	John Wiley and Sons Inc. 2016
Materias:	Communications
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5299463/ https://www.ncbi.nlm.nih.gov/pubmed/27966822 http://dx.doi.org/10.1002/anie.201608292

Ejemplares similares

Methods to Make Homogenous Antibody Drug Conjugates
por: Kline, Toni, et al.
Publicado: (2014)

Toward Homogenous Antibody Drug Conjugates Using Enzyme-Based Conjugation Approaches
por: Hussain, Ahmad Fawzi, et al.
Publicado: (2021)

In Vitro and In Vivo Evaluation of Cysteine and Site Specific Conjugated Herceptin Antibody-Drug Conjugates
por: Jackson, Dowdy, et al.
Publicado: (2014)

Dual-mechanistic antibody-drug conjugate via site-specific selenocysteine/cysteine conjugation
por: Nilchan, Napon, et al.
Publicado: (2019)

Acid-cleavable thiomaleamic acid linker for homogeneous antibody–drug conjugation
por: Castañeda, Lourdes, et al.
Publicado: (2013)

RF1 attenuation enables efficient non-natural amino acid incorporation for production of homogeneous antibody drug conjugates
por: Yin, Gang, et al.
Publicado: (2017)

Ethynylphosphonamidates for the Rapid and Cysteine‐Selective Generation of Efficacious Antibody–Drug Conjugates
por: Kasper, Marc‐André, et al.
Publicado: (2019)

Influence of disulfide bond isoforms on drug conjugation sites in cysteine-linked IgG2 antibody-drug conjugates
por: Liu-Shin, Lily, et al.
Publicado: (2018)

Construction of homogeneous antibody–drug conjugates using site-selective protein chemistry
por: Akkapeddi, Padma, et al.
Publicado: (2016)

Development of a facile antibody–drug conjugate platform for increased stability and homogeneity
por: Gupta, Nimish, et al.
Publicado: (2017)

Homogeneity of antibody-drug conjugates critically impacts the therapeutic efficacy in brain tumors
por: Anami, Yasuaki, et al.
Publicado: (2022)

A Versatile Chemo-Enzymatic Conjugation Approach Yields Homogeneous and Highly Potent Antibody-Drug Conjugates
por: Xu, Ying, et al.
Publicado: (2017)

Platform for Orthogonal N-Cysteine-Specific Protein Modification Enabled by Cyclopropenone Reagents
por: Istrate, Alena, et al.
Publicado: (2022)

A homogeneous high-DAR antibody–drug conjugate platform combining THIOMAB antibodies and XTEN polypeptides
por: Zacharias, Neelie, et al.
Publicado: (2022)

Interrogating heterogeneity of cysteine-engineered antibody-drug conjugates and antibody-oligonucleotide conjugates by capillary zone electrophoresis-mass spectrometry
por: Xu, Tian, et al.
Publicado: (2023)

Use of a charge reducing agent to enable intact mass analysis of cysteine-linked antibody-drug-conjugates by native mass spectrometry
por: Pacholarz, Kamila J., et al.
Publicado: (2016)

Design and Validation of Linkers for Site-Specific Preparation of Antibody–Drug Conjugates Carrying Multiple Drug Copies Per Cysteine Conjugation Site
por: Kumar, Amit, et al.
Publicado: (2020)

Cysteine metabolic engineering and selective disulfide reduction produce superior antibody-drug-conjugates
por: Procopio-Melino, Renée, et al.
Publicado: (2022)

Characterizing and understanding the formation of cysteine conjugates and other by-products in a random, lysine-linked antibody drug conjugate
por: Qiu, Difei, et al.
Publicado: (2021)

Improving Antibody‐Tubulysin Conjugates through Linker Chemistry and Site‐Specific Conjugation
por: Hamilton, Joseph Z., et al.
Publicado: (2021)

Harnessing a catalytic lysine residue for the one-step preparation of homogeneous antibody-drug conjugates
por: Nanna, Alex R., et al.
Publicado: (2017)

Investigating the Impact of Sample Preparation on Mass Spectrometry-Based Drug-To-Antibody Ratio Determination for Cysteine- and Lysine-Linked Antibody–Drug Conjugates
por: Källsten, Malin, et al.
Publicado: (2020)

Cysteine-to-lysine transfer antibody fragment conjugation
por: Forte, Nafsika, et al.
Publicado: (2019)

Antibody–Drug Conjugates for the Treatment of Acute Pediatric Leukemia
por: Stokke, Jamie L., et al.
Publicado: (2021)

A rapid on-line method for mass spectrometric confirmation of a cysteine-conjugated antibody-drug-conjugate structure using multidimensional chromatography
por: Birdsall, Robert E, et al.
Publicado: (2015)

An Innovative Site-Specific Anti-HER2 Antibody-Drug Conjugate with High Homogeneity and Improved Therapeutic Index
por: Hui, Xiwu, et al.
Publicado: (2022)

Native size-exclusion chromatography-mass spectrometry: suitability for antibody–drug conjugate drug-to-antibody ratio quantitation across a range of chemotypes and drug-loading levels
por: Jones, Jay, et al.
Publicado: (2019)

Intra-Domain Cysteines (IDC), a New Strategy for the Development of Original Antibody Fragment–Drug Conjugates (FDCs)
por: Jolivet, Louis, et al.
Publicado: (2022)

Homogeneous antibody-drug conjugates: DAR 2 anti-HER2 obtained by conjugation on isolated light chain followed by mAb assembly
por: Farràs, Mercè, et al.
Publicado: (2019)

Multivalent peptidic linker enables identification of preferred sites of conjugation for a potent thialanstatin antibody drug conjugate
por: Puthenveetil, Sujiet, et al.
Publicado: (2017)

Hydrophilic Auristatin Glycoside Payload Enables Improved Antibody-Drug Conjugate Efficacy and Biocompatibility
por: Satomaa, Tero, et al.
Publicado: (2018)

Galactose Oxidase Enables Modular Assembly of Conjugates from Native Antibodies with High Drug‐to‐Antibody Ratios
por: Angelastro, Antonio, et al.
Publicado: (2022)

Antibody-Oligonucleotide Conjugates: A Twist to Antibody-Drug Conjugates
por: Dugal-Tessier, Julien, et al.
Publicado: (2021)

Introduction to Antibody-Drug Conjugates
por: Pettinato, Mark C.
Publicado: (2021)

ANTIBODY DRUG CONJUGATES (ADCS)
por: Terpos, E.
Publicado: (2023)

Homogeneous antibody fragment conjugation by disulfide bridging introduces ‘spinostics'
por: Schumacher, Felix F., et al.
Publicado: (2013)

Homogeneous antibody–angiopep 2 conjugates for effective brain targeting
por: Anami, Yasuaki, et al.
Publicado: (2022)

State-of-the-Art Native Mass Spectrometry and Ion Mobility Methods to Monitor Homogeneous Site-Specific Antibody-Drug Conjugates Synthesis
por: Deslignière, Evolène, et al.
Publicado: (2021)

Site-Specific Antibody Conjugation to Engineered Double Cysteine Residues
por: Zhou, Qun, et al.
Publicado: (2021)

Antibody-Drug Conjugates in Bladder Cancer
por: Vlachostergios, Panagiotis J., et al.
Publicado: (2018)

Cannot write session to /tmp/vufind_sessions/sess_blrscsjqvsmq3eh3acl687rges