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Imaging specific newly synthesized proteins within cells by fluorescence resonance energy transfer

Metabolic azide amino acid labelling followed by the use of bioorthogonal chemistry is an efficient technique for imaging newly synthesized proteins. Recently, AHA-labelling together with the proximity-ligation assay was used to identify newly synthesized proteins of interest (POI) (Tom Dieck et al....

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Detalles Bibliográficos
Autores principales: Sheng, Linfeng, Cai, Lesi, Liu, Jie, Zhang, Sichun, Xu, Jing-Juan, Zhang, Xinrong, Chen, Hong-Yuan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Royal Society of Chemistry 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5299820/
https://www.ncbi.nlm.nih.gov/pubmed/28451223
http://dx.doi.org/10.1039/c6sc02610a