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Distinct effects of endosomal escape and inhibition of endosomal trafficking on gene delivery via electrotransfection

A recent theory suggests that endocytosis is involved in uptake and intracellular transport of electrotransfected plasmid DNA (pDNA). The goal of the current study was to understand if approaches used previously to improve endocytosis of gene delivery vectors could be applied to enhancing electrotra...

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Autores principales: Cervia, Lisa D., Chang, Chun-Chi, Wang, Liangli, Yuan, Fan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5300164/
https://www.ncbi.nlm.nih.gov/pubmed/28182739
http://dx.doi.org/10.1371/journal.pone.0171699
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author Cervia, Lisa D.
Chang, Chun-Chi
Wang, Liangli
Yuan, Fan
author_facet Cervia, Lisa D.
Chang, Chun-Chi
Wang, Liangli
Yuan, Fan
author_sort Cervia, Lisa D.
collection PubMed
description A recent theory suggests that endocytosis is involved in uptake and intracellular transport of electrotransfected plasmid DNA (pDNA). The goal of the current study was to understand if approaches used previously to improve endocytosis of gene delivery vectors could be applied to enhancing electrotransfection efficiency (eTE). Results from the study showed that photochemically induced endosomal escape, which could increase poly-L-lysine (PLL)-mediated gene delivery, decreased eTE. The decrease could not be blocked by treatment of cells with endonuclease inhibitors (aurintricarboxylic acid and zinc ion) or antioxidants (L-glutamine and ascorbic acid). Chemical treatment of cells with an endosomal trafficking inhibitor that blocks endosome progression, bafilomycin A1, resulted in a significant decrease in eTE. However, treatment of cells with lysosomotropic agents (chloroquine and ammonium chloride) had little effects on eTE. These data suggested that endosomes played important roles in protecting and intracellular trafficking of electrotransfected pDNA.
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spelling pubmed-53001642017-02-28 Distinct effects of endosomal escape and inhibition of endosomal trafficking on gene delivery via electrotransfection Cervia, Lisa D. Chang, Chun-Chi Wang, Liangli Yuan, Fan PLoS One Research Article A recent theory suggests that endocytosis is involved in uptake and intracellular transport of electrotransfected plasmid DNA (pDNA). The goal of the current study was to understand if approaches used previously to improve endocytosis of gene delivery vectors could be applied to enhancing electrotransfection efficiency (eTE). Results from the study showed that photochemically induced endosomal escape, which could increase poly-L-lysine (PLL)-mediated gene delivery, decreased eTE. The decrease could not be blocked by treatment of cells with endonuclease inhibitors (aurintricarboxylic acid and zinc ion) or antioxidants (L-glutamine and ascorbic acid). Chemical treatment of cells with an endosomal trafficking inhibitor that blocks endosome progression, bafilomycin A1, resulted in a significant decrease in eTE. However, treatment of cells with lysosomotropic agents (chloroquine and ammonium chloride) had little effects on eTE. These data suggested that endosomes played important roles in protecting and intracellular trafficking of electrotransfected pDNA. Public Library of Science 2017-02-09 /pmc/articles/PMC5300164/ /pubmed/28182739 http://dx.doi.org/10.1371/journal.pone.0171699 Text en © 2017 Cervia et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Cervia, Lisa D.
Chang, Chun-Chi
Wang, Liangli
Yuan, Fan
Distinct effects of endosomal escape and inhibition of endosomal trafficking on gene delivery via electrotransfection
title Distinct effects of endosomal escape and inhibition of endosomal trafficking on gene delivery via electrotransfection
title_full Distinct effects of endosomal escape and inhibition of endosomal trafficking on gene delivery via electrotransfection
title_fullStr Distinct effects of endosomal escape and inhibition of endosomal trafficking on gene delivery via electrotransfection
title_full_unstemmed Distinct effects of endosomal escape and inhibition of endosomal trafficking on gene delivery via electrotransfection
title_short Distinct effects of endosomal escape and inhibition of endosomal trafficking on gene delivery via electrotransfection
title_sort distinct effects of endosomal escape and inhibition of endosomal trafficking on gene delivery via electrotransfection
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5300164/
https://www.ncbi.nlm.nih.gov/pubmed/28182739
http://dx.doi.org/10.1371/journal.pone.0171699
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