Engineering the residual side chains of HAP phytases to improve their pepsin resistance and catalytic efficiency

Strong resistance to proteolytic attack is important for feed enzymes. Here, we selected three predicted pepsin cleavage sites, L99, L162, and E230 (numbering from the initiator M of premature proteins), in pepsin-sensitive HAP phytases YkAPPA from Yersinia kristensenii and YeAPPA from Y. enterocoli...

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Detalles Bibliográficos
Autores principales: Niu, Canfang, Yang, Peilong, Luo, Huiying, Huang, Huoqing, Wang, Yaru, Yao, Bin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5301473/
https://www.ncbi.nlm.nih.gov/pubmed/28186144
http://dx.doi.org/10.1038/srep42133

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5301473/
https://www.ncbi.nlm.nih.gov/pubmed/28186144
http://dx.doi.org/10.1038/srep42133

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