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Carotenoids from Foods of Plant, Animal and Marine Origin: An Efficient HPLC-DAD Separation Method

Carotenoids are important antioxidant compounds, present in many foods of plant, animal and marine origin. The aim of the present study was to describe the carotenoid composition of tomato waste, prawn muscle and cephalothorax and avian (duck and goose) egg yolks through the use of a modified gradie...

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Autores principales: Strati, Irini F., Sinanoglou, Vassilia J., Kora, Lintita, Miniadis-Meimaroglou, Sofia, Oreopoulou, Vassiliki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5302218/
https://www.ncbi.nlm.nih.gov/pubmed/28239091
http://dx.doi.org/10.3390/foods1010052
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author Strati, Irini F.
Sinanoglou, Vassilia J.
Kora, Lintita
Miniadis-Meimaroglou, Sofia
Oreopoulou, Vassiliki
author_facet Strati, Irini F.
Sinanoglou, Vassilia J.
Kora, Lintita
Miniadis-Meimaroglou, Sofia
Oreopoulou, Vassiliki
author_sort Strati, Irini F.
collection PubMed
description Carotenoids are important antioxidant compounds, present in many foods of plant, animal and marine origin. The aim of the present study was to describe the carotenoid composition of tomato waste, prawn muscle and cephalothorax and avian (duck and goose) egg yolks through the use of a modified gradient elution HPLC method with a C(30) reversed-phase column for the efficient separation and analysis of carotenoids and their cis-isomers. Elution time was reduced from 60 to 45 min without affecting the separation efficiency. All-trans lycopene predominated in tomato waste, followed by all-trans-β-carotene, 13-cis-lutein and all-trans lutein, while minor amounts of 9-cis-lutein, 13-cis-β-carotene and 9-cis-β-carotene were also detected. Considering the above findings, tomato waste is confirmed to be an excellent source of recovering carotenoids, especially all-trans lycopene, for commercial use. Xanthophylls were the major carotenoids of avian egg yolks, all-trans lutein and all-trans zeaxanthin in duck and goose egg yolk, respectively. In the Penaeus kerathurus prawn, several carotenoids (zeaxanthin, all-trans-lutein, canthaxanthin, cryptoxanthin, optical and geometrical astaxanthin isomers) were identified in considerable amounts by the same method. A major advantage of this HPLC method was the efficient separation of carotenoids and their cis-isomers, originating from a wide range of matrices.
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spelling pubmed-53022182017-02-15 Carotenoids from Foods of Plant, Animal and Marine Origin: An Efficient HPLC-DAD Separation Method Strati, Irini F. Sinanoglou, Vassilia J. Kora, Lintita Miniadis-Meimaroglou, Sofia Oreopoulou, Vassiliki Foods Article Carotenoids are important antioxidant compounds, present in many foods of plant, animal and marine origin. The aim of the present study was to describe the carotenoid composition of tomato waste, prawn muscle and cephalothorax and avian (duck and goose) egg yolks through the use of a modified gradient elution HPLC method with a C(30) reversed-phase column for the efficient separation and analysis of carotenoids and their cis-isomers. Elution time was reduced from 60 to 45 min without affecting the separation efficiency. All-trans lycopene predominated in tomato waste, followed by all-trans-β-carotene, 13-cis-lutein and all-trans lutein, while minor amounts of 9-cis-lutein, 13-cis-β-carotene and 9-cis-β-carotene were also detected. Considering the above findings, tomato waste is confirmed to be an excellent source of recovering carotenoids, especially all-trans lycopene, for commercial use. Xanthophylls were the major carotenoids of avian egg yolks, all-trans lutein and all-trans zeaxanthin in duck and goose egg yolk, respectively. In the Penaeus kerathurus prawn, several carotenoids (zeaxanthin, all-trans-lutein, canthaxanthin, cryptoxanthin, optical and geometrical astaxanthin isomers) were identified in considerable amounts by the same method. A major advantage of this HPLC method was the efficient separation of carotenoids and their cis-isomers, originating from a wide range of matrices. MDPI 2012-12-19 /pmc/articles/PMC5302218/ /pubmed/28239091 http://dx.doi.org/10.3390/foods1010052 Text en © 2012 by the authors; licensee MDPI, Basel, Switzerland. This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Strati, Irini F.
Sinanoglou, Vassilia J.
Kora, Lintita
Miniadis-Meimaroglou, Sofia
Oreopoulou, Vassiliki
Carotenoids from Foods of Plant, Animal and Marine Origin: An Efficient HPLC-DAD Separation Method
title Carotenoids from Foods of Plant, Animal and Marine Origin: An Efficient HPLC-DAD Separation Method
title_full Carotenoids from Foods of Plant, Animal and Marine Origin: An Efficient HPLC-DAD Separation Method
title_fullStr Carotenoids from Foods of Plant, Animal and Marine Origin: An Efficient HPLC-DAD Separation Method
title_full_unstemmed Carotenoids from Foods of Plant, Animal and Marine Origin: An Efficient HPLC-DAD Separation Method
title_short Carotenoids from Foods of Plant, Animal and Marine Origin: An Efficient HPLC-DAD Separation Method
title_sort carotenoids from foods of plant, animal and marine origin: an efficient hplc-dad separation method
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5302218/
https://www.ncbi.nlm.nih.gov/pubmed/28239091
http://dx.doi.org/10.3390/foods1010052
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