Effect of Pre-Harvest Sprouting on Physicochemical Properties of Starch in Wheat

Pre-harvest sprouting (PHS) in wheat (Triticum aestivum L.) occurs when physiologically mature kernels begin germinating in the spike. The objective of this study was to provide fundamental information on physicochemical changes of starch due to PHS in Hard Red Spring (HRS) and Hard White Spring (HW...

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Autores principales: Simsek, Senay, Ohm, Jae-Bom, Lu, Haiyan, Rugg, Mory, Berzonsky, William, Alamri, Mohammed S., Mergoum, Mohamed
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2014
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5302366/
https://www.ncbi.nlm.nih.gov/pubmed/28234313
http://dx.doi.org/10.3390/foods3020194
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author Simsek, Senay
Ohm, Jae-Bom
Lu, Haiyan
Rugg, Mory
Berzonsky, William
Alamri, Mohammed S.
Mergoum, Mohamed
author_facet Simsek, Senay
Ohm, Jae-Bom
Lu, Haiyan
Rugg, Mory
Berzonsky, William
Alamri, Mohammed S.
Mergoum, Mohamed
author_sort Simsek, Senay
collection PubMed
description Pre-harvest sprouting (PHS) in wheat (Triticum aestivum L.) occurs when physiologically mature kernels begin germinating in the spike. The objective of this study was to provide fundamental information on physicochemical changes of starch due to PHS in Hard Red Spring (HRS) and Hard White Spring (HWS) wheat. The mean values of α-amylase activity of non-sprouted and sprouted wheat samples were 0.12 CU/g and 2.00 CU/g, respectively. Sprouted samples exhibited very low peak and final viscosities compared to non-sprouted wheat samples. Scanning electron microscopy (SEM) images showed that starch granules in sprouted samples were partially hydrolyzed. Based on High Performance Size Exclusion Chromatography (HPSEC) profiles, the starch from sprouted samples had relatively lower molecular weight than that of non-sprouted samples. Overall, high α-amylase activity caused changes to the physicochemical properties of the PHS damaged wheat.
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spelling pubmed-53023662017-02-15 Effect of Pre-Harvest Sprouting on Physicochemical Properties of Starch in Wheat Simsek, Senay Ohm, Jae-Bom Lu, Haiyan Rugg, Mory Berzonsky, William Alamri, Mohammed S. Mergoum, Mohamed Foods Article Pre-harvest sprouting (PHS) in wheat (Triticum aestivum L.) occurs when physiologically mature kernels begin germinating in the spike. The objective of this study was to provide fundamental information on physicochemical changes of starch due to PHS in Hard Red Spring (HRS) and Hard White Spring (HWS) wheat. The mean values of α-amylase activity of non-sprouted and sprouted wheat samples were 0.12 CU/g and 2.00 CU/g, respectively. Sprouted samples exhibited very low peak and final viscosities compared to non-sprouted wheat samples. Scanning electron microscopy (SEM) images showed that starch granules in sprouted samples were partially hydrolyzed. Based on High Performance Size Exclusion Chromatography (HPSEC) profiles, the starch from sprouted samples had relatively lower molecular weight than that of non-sprouted samples. Overall, high α-amylase activity caused changes to the physicochemical properties of the PHS damaged wheat. MDPI 2014-04-08 /pmc/articles/PMC5302366/ /pubmed/28234313 http://dx.doi.org/10.3390/foods3020194 Text en © 2014 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Simsek, Senay
Ohm, Jae-Bom
Lu, Haiyan
Rugg, Mory
Berzonsky, William
Alamri, Mohammed S.
Mergoum, Mohamed
Effect of Pre-Harvest Sprouting on Physicochemical Properties of Starch in Wheat
title Effect of Pre-Harvest Sprouting on Physicochemical Properties of Starch in Wheat
title_full Effect of Pre-Harvest Sprouting on Physicochemical Properties of Starch in Wheat
title_fullStr Effect of Pre-Harvest Sprouting on Physicochemical Properties of Starch in Wheat
title_full_unstemmed Effect of Pre-Harvest Sprouting on Physicochemical Properties of Starch in Wheat
title_short Effect of Pre-Harvest Sprouting on Physicochemical Properties of Starch in Wheat
title_sort effect of pre-harvest sprouting on physicochemical properties of starch in wheat
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5302366/
https://www.ncbi.nlm.nih.gov/pubmed/28234313
http://dx.doi.org/10.3390/foods3020194
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