Significant impact of amount of PCR input templates on various PCR-based DNA methylation analysis and countermeasure

Methylation changes of CpG islands can be determined using PCR-based assays. However, the exact impact of the amount of input templates (TAIT) on DNA methylation analysis has not been previously recognized. Using COL2A1 gene as an input reference, TAIT difference between human tissues with methylati...

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Detalles Bibliográficos
Autores principales: Liu, Zhaojun, Zhou, Jing, Gu, Liankun, Deng, Dajun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals LLC 2016
Materias:
Research Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5302926/
https://www.ncbi.nlm.nih.gov/pubmed/27487147
http://dx.doi.org/10.18632/oncotarget.10906

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5302926/
https://www.ncbi.nlm.nih.gov/pubmed/27487147
http://dx.doi.org/10.18632/oncotarget.10906

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