Cargando…

Significant impact of amount of PCR input templates on various PCR-based DNA methylation analysis and countermeasure

Methylation changes of CpG islands can be determined using PCR-based assays. However, the exact impact of the amount of input templates (TAIT) on DNA methylation analysis has not been previously recognized. Using COL2A1 gene as an input reference, TAIT difference between human tissues with methylati...

Descripción completa

Detalles Bibliográficos
Autores principales:	Liu, Zhaojun, Zhou, Jing, Gu, Liankun, Deng, Dajun
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Impact Journals LLC 2016
Materias:	Research Paper
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5302926/ https://www.ncbi.nlm.nih.gov/pubmed/27487147 http://dx.doi.org/10.18632/oncotarget.10906

Ejemplares similares

Detection of somatic copy number deletion of the CDKN2A gene by quantitative multiplex PCR for clinical practice
por: Tian, Yuan, et al.
Publicado: (2022)

P16-specific DNA methylation by engineered zinc finger methyltransferase inactivates gene transcription and promotes cancer metastasis
por: Cui, Chenghua, et al.
Publicado: (2015)

Characterization of human gastric carcinoma-related methylation of 9 miR CpG islands and repression of their expressions in vitro and in vivo
por: Du, Yantao, et al.
Publicado: (2012)

Improved PCR Amplification of Broad Spectrum GC DNA Templates
por: Guido, Nicholas, et al.
Publicado: (2016)

P16 Methylation Leads to Paclitaxel Resistance of Advanced Non-Small Cell Lung Cancer
por: Liu, Zhaojun, et al.
Publicado: (2019)

SRF promotes gastric cancer metastasis through stromal fibroblasts in an SDF1-CXCR4-dependent manner
por: Qiao, Juanli, et al.
Publicado: (2016)

P16 methylation increases the sensitivity of cancer cells to the CDK4/6 inhibitor palbociclib
por: Li, Paiyun, et al.
Publicado: (2019)

High-throughput stem-loop RT-qPCR miRNA expression profiling using minute amounts of input RNA
por: Mestdagh, Pieter, et al.
Publicado: (2008)

Improved PCR Performance Using Template DNA from Formalin-Fixed and Paraffin-Embedded Tissues by Overcoming PCR Inhibition
por: Dietrich, Dimo, et al.
Publicado: (2013)

Chidamide increases the sensitivity of Non-small Cell Lung Cancer to Crizotinib by decreasing c-MET mRNA methylation
por: Ding, Nan, et al.
Publicado: (2020)

Methods for Multiplex Template Sampling in Digital PCR Assays
por: Petriv, Oleh I., et al.
Publicado: (2014)

A simple and rapid technique of template preparation for PCR
por: Liu, Yunyun, et al.
Publicado: (2022)

MSRE-PCR for analysis of gene-specific DNA methylation
por: Melnikov, Anatoliy A., et al.
Publicado: (2005)

De Novo synthesis of PCR templates for the development of SARS diagnostic assay
por: Zhang, Jia, et al.
Publicado: (2003)

TqPCR: A Touchdown qPCR Assay with Significantly Improved Detection Sensitivity and Amplification Efficiency of SYBR Green qPCR
por: Zhang, Qian, et al.
Publicado: (2015)

Indexed PCR Primers Induce Template-Specific Bias in Large-Scale DNA Sequencing Studies
por: O’Donnell, James L., et al.
Publicado: (2016)

C-terminal in Sp1-like artificial zinc-finger proteins plays crucial roles in determining their DNA binding affinity
por: Zhang, Baozhen, et al.
Publicado: (2013)

Fluorescent-increase kinetics of different fluorescent reporters used for qPCR depend on monitoring chemistry, targeted sequence, type of DNA input and PCR efficiency
por: Ruijter, Jan M., et al.
Publicado: (2014)

NGS-based identification and tracing of microsatellite instability from minute amounts DNA using inter-Alu-PCR
por: Yu, Fangyan, et al.
Publicado: (2020)

Parallel DNA polymerase chain reaction: Synthesis of two different PCR products from a DNA template
por: Bhardwaj, Vikash, et al.
Publicado: (2014)

Statistical significance of quantitative PCR
por: Karlen, Yann, et al.
Publicado: (2007)

Bone Marrow-Derived Cells May Not Be the Original Cells for Carcinogen-Induced Mouse Gastrointestinal Carcinomas
por: Yang, Chen, et al.
Publicado: (2013)

Estimating the number of contributors to two-, three-, and four-person mixtures containing DNA in high template and low template amounts
por: Perez, Jaheida, et al.
Publicado: (2011)

Rapid Site-Directed Mutagenesis Using Two-PCR-Generated DNA Fragments Reproducing the Plasmid Template
por: Allemandou, Flore, et al.
Publicado: (2003)

Methylation-Specific PCR Unraveled
por: Derks, Sarah, et al.
Publicado: (2004)

Methodology for Detecting Trace Amounts of Microchimeric DNA from Peripheral Murine White Blood Cells by Real-Time PCR
por: Artlett, Carol M., et al.
Publicado: (2003)

A panel of DNA methylated markers predicts metastasis of pN(0)M(0) gastric carcinoma: a prospective cohort study
por: Liu, Zhaojun, et al.
Publicado: (2019)

Critical evaluation of Cbx7 downregulation in primary colon carcinomas and its clinical significance in Chinese patients
por: Zheng, Xiang, et al.
Publicado: (2015)

Be aware of the allele-specific bias and compositional effects in multi-template PCR
por: Korvigo, Ilia, et al.
Publicado: (2022)

The use of Multiple Displacement Amplified DNA as a control for Methylation Specific PCR, Pyrosequencing, Bisulfite Sequencing and Methylation-Sensitive Restriction Enzyme PCR
por: Hughes, Simon, et al.
Publicado: (2007)

Different DNA methylation patterns detected by the Amplified Methylation Polymorphism Polymerase Chain Reaction (AMP PCR) technique among various cell types of bulls
por: Phutikanit, Nawapen, et al.
Publicado: (2010)

A similar effect of P16 hydroxymethylation and true-methylation on the prediction of malignant transformation of oral epithelial dysplasia: observation from a prospective study
por: Liu, Hongwei, et al.
Publicado: (2018)

Total DNA input is a crucial determinant of the sensitivity of plasma cell-free DNA EGFR mutation detection using droplet digital PCR
por: Zhang, Yu, et al.
Publicado: (2016)

A qPCR technology for direct quantification of methylation in untreated DNA
por: Bendixen, Kamilla Kolding, et al.
Publicado: (2023)

Characterization of novel LncRNA P14AS as a protector of ANRIL through AUF1 binding in human cells
por: Ma, Wanru, et al.
Publicado: (2020)

Comparison of Digital PCR and Quantitative PCR with Various SARS-CoV-2 Primer-Probe Sets
por: Park, Changwoo, et al.
Publicado: (2021)

Extensive Recombination Due to Heteroduplexes Generates Large Amounts of Artificial Gene Fragments during PCR
por: Liu, Jia, et al.
Publicado: (2014)

Generation of non-genomic oligonucleotide tag sequences for RNA template-specific PCR
por: Pinto, Fernando Lopes, et al.
Publicado: (2006)

A method for counting PCR template molecules with application to next-generation sequencing
por: Casbon, James A., et al.
Publicado: (2011)

Template copy number and the sensitivity of quantitative PCR for Plasmodium falciparum in asymptomatic individuals
por: Thompson, Trevor A., et al.
Publicado: (2020)

Cannot write session to /tmp/vufind_sessions/sess_h9k8k8o68jkeh7jfimlc157a1o