High-throughput in situ cell electroporation microsystem for parallel delivery of single guide RNAs into mammalian cells

Arrayed genetic screens mediated by the CRISPR/Cas9 technology with single guide RNA (sgRNA) libraries demand a high-throughput platform capable of transfecting diverse cell types at a high efficiency in a genome-wide scale for detection and analysis of sophisticated cellular phenotypes. Here we dev...

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Detalles Bibliográficos
Autores principales: Bian, Shengtai, Zhou, Yicen, Hu, Yawei, Cheng, Jing, Chen, Xiaofang, Xu, Youchun, Liu, Peng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5304186/
https://www.ncbi.nlm.nih.gov/pubmed/28211892
http://dx.doi.org/10.1038/srep42512

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5304186/
https://www.ncbi.nlm.nih.gov/pubmed/28211892
http://dx.doi.org/10.1038/srep42512

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