Rapid differentiation of human pluripotent stem cells into functional neurons by mRNAs encoding transcription factors

Efficient differentiation of human pluripotent stem cells (hPSCs) into neurons is paramount for disease modeling, drug screening, and cell transplantation therapy in regenerative medicine. In this manuscript, we report the capability of five transcription factors (TFs) toward this aim: NEUROG1, NEUR...

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Detalles Bibliográficos
Autores principales: Goparaju, Sravan Kumar, Kohda, Kazuhisa, Ibata, Keiji, Soma, Atsumi, Nakatake, Yukhi, Akiyama, Tomohiko, Wakabayashi, Shunichi, Matsushita, Misako, Sakota, Miki, Kimura, Hiromi, Yuzaki, Michisuke, Ko, Shigeru B. H., Ko, Minoru S. H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5304326/
https://www.ncbi.nlm.nih.gov/pubmed/28205555
http://dx.doi.org/10.1038/srep42367
Descripción
Sumario:Efficient differentiation of human pluripotent stem cells (hPSCs) into neurons is paramount for disease modeling, drug screening, and cell transplantation therapy in regenerative medicine. In this manuscript, we report the capability of five transcription factors (TFs) toward this aim: NEUROG1, NEUROG2, NEUROG3, NEUROD1, and NEUROD2. In contrast to previous methods that have shortcomings in their speed and efficiency, a cocktail of these TFs as synthetic mRNAs can differentiate hPSCs into neurons in 7 days, judged by calcium imaging and electrophysiology. They exhibit motor neuron phenotypes based on immunostaining. These results indicate the establishment of a novel method for rapid, efficient, and footprint-free differentiation of functional neurons from hPSCs.

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