Ocean acidification modulates expression of genes and physiological performance of a marine diatom

Ocean Acidification (OA) is known to affect various aspects of physiological performances of diatoms, but little is known about the underlining molecular mechanisms involved. Here, we show that in the model diatom Phaeodactylum tricornutum, the expression of key genes associated with photosynthetic...

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Autores principales: Li, Yahe, Zhuang, Shufang, Wu, Yaping, Ren, Honglin, Chen, Fangyi, Lin, Xin, Wang, Kejian, Beardall, John, Gao, Kunshan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5305191/
https://www.ncbi.nlm.nih.gov/pubmed/28192486
http://dx.doi.org/10.1371/journal.pone.0170970
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author Li, Yahe
Zhuang, Shufang
Wu, Yaping
Ren, Honglin
Chen, Fangyi
Lin, Xin
Wang, Kejian
Beardall, John
Gao, Kunshan
author_facet Li, Yahe
Zhuang, Shufang
Wu, Yaping
Ren, Honglin
Chen, Fangyi
Lin, Xin
Wang, Kejian
Beardall, John
Gao, Kunshan
author_sort Li, Yahe
collection PubMed
description Ocean Acidification (OA) is known to affect various aspects of physiological performances of diatoms, but little is known about the underlining molecular mechanisms involved. Here, we show that in the model diatom Phaeodactylum tricornutum, the expression of key genes associated with photosynthetic light harvesting as well as those encoding Rubisco, carbonic anhydrase, NADH dehydrogenase and nitrite reductase, are modulated by OA (1000 μatm, pH(nbs) 7.83). Growth and photosynthetic carbon fixation were enhanced by elevated CO(2). OA treatment decreased the expression of β-carbonic anhydrase (β-ca), which functions in balancing intracellular carbonate chemistry and the CO(2) concentrating mechanism (CCM). The expression of the genes encoding fucoxanthin chlorophyll a/c protein (lhcf type (fcp)), mitochondrial ATP synthase (mtATP), ribulose-1, 5-bisphosphate carboxylase/oxygenase large subunit gene (rbcl) and NADH dehydrogenase subunit 2 (ndh2), were down-regulated during the first four days (< 8 generations) after the cells were transferred from LC (cells grown under ambient air condition; 390 μatm; pH(nbs) 8.19) to OA conditions, with no significant difference between LC and HC treatments with the time elapsed. The expression of nitrite reductase (nir) was up-regulated by the OA treatment. Additionally, the genes for these proteins (NiR, FCP, mtATP synthase, β-CA) showed diel expression patterns. It appeared that the enhanced photosynthetic and growth rates under OA could be attributed to stimulated nitrogen assimilation, increased CO(2) availability or saved energy from down-regulation of the CCM and consequently lowered cost of protein synthesis versus that of non-nitrogenous cell components.
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spelling pubmed-53051912017-02-28 Ocean acidification modulates expression of genes and physiological performance of a marine diatom Li, Yahe Zhuang, Shufang Wu, Yaping Ren, Honglin Chen, Fangyi Lin, Xin Wang, Kejian Beardall, John Gao, Kunshan PLoS One Research Article Ocean Acidification (OA) is known to affect various aspects of physiological performances of diatoms, but little is known about the underlining molecular mechanisms involved. Here, we show that in the model diatom Phaeodactylum tricornutum, the expression of key genes associated with photosynthetic light harvesting as well as those encoding Rubisco, carbonic anhydrase, NADH dehydrogenase and nitrite reductase, are modulated by OA (1000 μatm, pH(nbs) 7.83). Growth and photosynthetic carbon fixation were enhanced by elevated CO(2). OA treatment decreased the expression of β-carbonic anhydrase (β-ca), which functions in balancing intracellular carbonate chemistry and the CO(2) concentrating mechanism (CCM). The expression of the genes encoding fucoxanthin chlorophyll a/c protein (lhcf type (fcp)), mitochondrial ATP synthase (mtATP), ribulose-1, 5-bisphosphate carboxylase/oxygenase large subunit gene (rbcl) and NADH dehydrogenase subunit 2 (ndh2), were down-regulated during the first four days (< 8 generations) after the cells were transferred from LC (cells grown under ambient air condition; 390 μatm; pH(nbs) 8.19) to OA conditions, with no significant difference between LC and HC treatments with the time elapsed. The expression of nitrite reductase (nir) was up-regulated by the OA treatment. Additionally, the genes for these proteins (NiR, FCP, mtATP synthase, β-CA) showed diel expression patterns. It appeared that the enhanced photosynthetic and growth rates under OA could be attributed to stimulated nitrogen assimilation, increased CO(2) availability or saved energy from down-regulation of the CCM and consequently lowered cost of protein synthesis versus that of non-nitrogenous cell components. Public Library of Science 2017-02-13 /pmc/articles/PMC5305191/ /pubmed/28192486 http://dx.doi.org/10.1371/journal.pone.0170970 Text en © 2017 Li et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Li, Yahe
Zhuang, Shufang
Wu, Yaping
Ren, Honglin
Chen, Fangyi
Lin, Xin
Wang, Kejian
Beardall, John
Gao, Kunshan
Ocean acidification modulates expression of genes and physiological performance of a marine diatom
title Ocean acidification modulates expression of genes and physiological performance of a marine diatom
title_full Ocean acidification modulates expression of genes and physiological performance of a marine diatom
title_fullStr Ocean acidification modulates expression of genes and physiological performance of a marine diatom
title_full_unstemmed Ocean acidification modulates expression of genes and physiological performance of a marine diatom
title_short Ocean acidification modulates expression of genes and physiological performance of a marine diatom
title_sort ocean acidification modulates expression of genes and physiological performance of a marine diatom
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5305191/
https://www.ncbi.nlm.nih.gov/pubmed/28192486
http://dx.doi.org/10.1371/journal.pone.0170970
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