Cargando…

Fidgetin regulates cultured astrocyte migration by severing tyrosinated microtubules at the leading edge

Microtubule (MT) organization is essential for many cellular events, including mitosis, migration, and cell polarity. Fidgetin (Fign), an ATP-dependent, MT-severing protein, contributes to the regulation of MT configuration by cutting and trimming MT polymers. Functions of Fign have been indicated i...

Descripción completa

Detalles Bibliográficos
Autores principales: Hu, Zunlu, Feng, Jie, Bo, Weijuan, Wu, Ronghua, Dong, Zhangji, Liu, Yan, Qiang, Liang, Liu, Mei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5305261/
https://www.ncbi.nlm.nih.gov/pubmed/27974640
http://dx.doi.org/10.1091/mbc.E16-09-0628
_version_ 1782507021499629568
author Hu, Zunlu
Feng, Jie
Bo, Weijuan
Wu, Ronghua
Dong, Zhangji
Liu, Yan
Qiang, Liang
Liu, Mei
author_facet Hu, Zunlu
Feng, Jie
Bo, Weijuan
Wu, Ronghua
Dong, Zhangji
Liu, Yan
Qiang, Liang
Liu, Mei
author_sort Hu, Zunlu
collection PubMed
description Microtubule (MT) organization is essential for many cellular events, including mitosis, migration, and cell polarity. Fidgetin (Fign), an ATP-dependent, MT-severing protein, contributes to the regulation of MT configuration by cutting and trimming MT polymers. Functions of Fign have been indicated in neurite outgrowth, mitosis, meiosis, and cellular migration. Here we focus on migration of astrocytes. We find that Fign plays an essential role in cultured astrocyte migration by preferentially targeting MTs (or regions of MTs) that are rich in tyrosinated tubulin, a marker for especially dynamic MTs or especially dynamic regions of MTs. Inhibition of cellular migration induced by Fign knockdown can be rescued with concomitant knockdown of kinesin-12, a motor protein best known for its role in mitosis. We propose a novel working model for MT reconfiguration underlying cellular migration elicited by the functional cooperation of two distinct MT-related proteins.
format Online
Article
Text
id pubmed-5305261
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher The American Society for Cell Biology
record_format MEDLINE/PubMed
spelling pubmed-53052612017-04-30 Fidgetin regulates cultured astrocyte migration by severing tyrosinated microtubules at the leading edge Hu, Zunlu Feng, Jie Bo, Weijuan Wu, Ronghua Dong, Zhangji Liu, Yan Qiang, Liang Liu, Mei Mol Biol Cell Articles Microtubule (MT) organization is essential for many cellular events, including mitosis, migration, and cell polarity. Fidgetin (Fign), an ATP-dependent, MT-severing protein, contributes to the regulation of MT configuration by cutting and trimming MT polymers. Functions of Fign have been indicated in neurite outgrowth, mitosis, meiosis, and cellular migration. Here we focus on migration of astrocytes. We find that Fign plays an essential role in cultured astrocyte migration by preferentially targeting MTs (or regions of MTs) that are rich in tyrosinated tubulin, a marker for especially dynamic MTs or especially dynamic regions of MTs. Inhibition of cellular migration induced by Fign knockdown can be rescued with concomitant knockdown of kinesin-12, a motor protein best known for its role in mitosis. We propose a novel working model for MT reconfiguration underlying cellular migration elicited by the functional cooperation of two distinct MT-related proteins. The American Society for Cell Biology 2017-02-15 /pmc/articles/PMC5305261/ /pubmed/27974640 http://dx.doi.org/10.1091/mbc.E16-09-0628 Text en © 2017 Hu et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.
spellingShingle Articles
Hu, Zunlu
Feng, Jie
Bo, Weijuan
Wu, Ronghua
Dong, Zhangji
Liu, Yan
Qiang, Liang
Liu, Mei
Fidgetin regulates cultured astrocyte migration by severing tyrosinated microtubules at the leading edge
title Fidgetin regulates cultured astrocyte migration by severing tyrosinated microtubules at the leading edge
title_full Fidgetin regulates cultured astrocyte migration by severing tyrosinated microtubules at the leading edge
title_fullStr Fidgetin regulates cultured astrocyte migration by severing tyrosinated microtubules at the leading edge
title_full_unstemmed Fidgetin regulates cultured astrocyte migration by severing tyrosinated microtubules at the leading edge
title_short Fidgetin regulates cultured astrocyte migration by severing tyrosinated microtubules at the leading edge
title_sort fidgetin regulates cultured astrocyte migration by severing tyrosinated microtubules at the leading edge
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5305261/
https://www.ncbi.nlm.nih.gov/pubmed/27974640
http://dx.doi.org/10.1091/mbc.E16-09-0628
work_keys_str_mv AT huzunlu fidgetinregulatesculturedastrocytemigrationbyseveringtyrosinatedmicrotubulesattheleadingedge
AT fengjie fidgetinregulatesculturedastrocytemigrationbyseveringtyrosinatedmicrotubulesattheleadingedge
AT boweijuan fidgetinregulatesculturedastrocytemigrationbyseveringtyrosinatedmicrotubulesattheleadingedge
AT wuronghua fidgetinregulatesculturedastrocytemigrationbyseveringtyrosinatedmicrotubulesattheleadingedge
AT dongzhangji fidgetinregulatesculturedastrocytemigrationbyseveringtyrosinatedmicrotubulesattheleadingedge
AT liuyan fidgetinregulatesculturedastrocytemigrationbyseveringtyrosinatedmicrotubulesattheleadingedge
AT qiangliang fidgetinregulatesculturedastrocytemigrationbyseveringtyrosinatedmicrotubulesattheleadingedge
AT liumei fidgetinregulatesculturedastrocytemigrationbyseveringtyrosinatedmicrotubulesattheleadingedge