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Polymerase cross-linking spiral reaction (PCLSR) for detection of African swine fever virus (ASFV) in pigs and wild boars

The study reports the development of a polymerase cross-linking spiral reaction (PCLSR) for the detection of African swine fever virus (ASFV) DNA in blood collected from infected pigs and wild boars. The method uses 3 specifically designed primers. Two outer-spiral primers comprising of 3′ sequences...

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Detalles Bibliográficos
Autores principales: Woźniakowski, Grzegorz, Frączyk, Magdalena, Kowalczyk, Andrzej, Pomorska-Mól, Małgorzata, Niemczuk, Krzysztof, Pejsak, Zygmunt
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5309890/
https://www.ncbi.nlm.nih.gov/pubmed/28198455
http://dx.doi.org/10.1038/srep42903