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Oleandrin induces DNA damage responses in cancer cells by suppressing the expression of Rad51
Oleandrin is a monomeric compound extracted from leaves and seeds of Nerium oleander. It had been reported that oleandrin could effectively inhibit the growth of human cancer cells. However, the specific mechanisms of the oleandrin-induced anti-tumor effects remain largely unclear. Genomic instabili...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Impact Journals LLC
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5312332/ https://www.ncbi.nlm.nih.gov/pubmed/27449097 http://dx.doi.org/10.18632/oncotarget.10726 |
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author | Bao, Zhengqiang Tian, Baoping Wang, Xiaohui Feng, Hanrong Liang, Ye Chen, Zhihua Li, Wen Shen, Huahao Ying, Songmin |
author_facet | Bao, Zhengqiang Tian, Baoping Wang, Xiaohui Feng, Hanrong Liang, Ye Chen, Zhihua Li, Wen Shen, Huahao Ying, Songmin |
author_sort | Bao, Zhengqiang |
collection | PubMed |
description | Oleandrin is a monomeric compound extracted from leaves and seeds of Nerium oleander. It had been reported that oleandrin could effectively inhibit the growth of human cancer cells. However, the specific mechanisms of the oleandrin-induced anti-tumor effects remain largely unclear. Genomic instability is one of the main features of cancer cells, it can be the combined effect of DNA damage and tumour-specific DNA repair defects. DNA damage plays important roles during tumorigenesis. In fact, most of the current chemotherapy agents were designed to kill cancer cells by inducing DNA damage. In this study, we found that oleandrin was effective to induce apoptosis in cancer cells, and cause rapid DNA damage response, represented by nuclear RPA (Replication Protein A, a single strand DNA binding protein) and γH2AX(a marker for DNA double strand breaks) foci formation. Interestingly, expression of RAD51, a key protein involved in homologous recombination (HR), was suppressed while XRCC1 was up-regulated in oleandrin treated cancer cells. These results suggested that XRCC1 may play a predominant role in repairing oleandrin-induced DNA damage. Collectively, oleandrin may be a potential anti-tumor agent by suppressing the expression of Rad51. |
format | Online Article Text |
id | pubmed-5312332 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Impact Journals LLC |
record_format | MEDLINE/PubMed |
spelling | pubmed-53123322017-03-06 Oleandrin induces DNA damage responses in cancer cells by suppressing the expression of Rad51 Bao, Zhengqiang Tian, Baoping Wang, Xiaohui Feng, Hanrong Liang, Ye Chen, Zhihua Li, Wen Shen, Huahao Ying, Songmin Oncotarget Research Paper Oleandrin is a monomeric compound extracted from leaves and seeds of Nerium oleander. It had been reported that oleandrin could effectively inhibit the growth of human cancer cells. However, the specific mechanisms of the oleandrin-induced anti-tumor effects remain largely unclear. Genomic instability is one of the main features of cancer cells, it can be the combined effect of DNA damage and tumour-specific DNA repair defects. DNA damage plays important roles during tumorigenesis. In fact, most of the current chemotherapy agents were designed to kill cancer cells by inducing DNA damage. In this study, we found that oleandrin was effective to induce apoptosis in cancer cells, and cause rapid DNA damage response, represented by nuclear RPA (Replication Protein A, a single strand DNA binding protein) and γH2AX(a marker for DNA double strand breaks) foci formation. Interestingly, expression of RAD51, a key protein involved in homologous recombination (HR), was suppressed while XRCC1 was up-regulated in oleandrin treated cancer cells. These results suggested that XRCC1 may play a predominant role in repairing oleandrin-induced DNA damage. Collectively, oleandrin may be a potential anti-tumor agent by suppressing the expression of Rad51. Impact Journals LLC 2016-07-20 /pmc/articles/PMC5312332/ /pubmed/27449097 http://dx.doi.org/10.18632/oncotarget.10726 Text en Copyright: © 2016 Bao et al. http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Paper Bao, Zhengqiang Tian, Baoping Wang, Xiaohui Feng, Hanrong Liang, Ye Chen, Zhihua Li, Wen Shen, Huahao Ying, Songmin Oleandrin induces DNA damage responses in cancer cells by suppressing the expression of Rad51 |
title | Oleandrin induces DNA damage responses in cancer cells by suppressing the expression of Rad51 |
title_full | Oleandrin induces DNA damage responses in cancer cells by suppressing the expression of Rad51 |
title_fullStr | Oleandrin induces DNA damage responses in cancer cells by suppressing the expression of Rad51 |
title_full_unstemmed | Oleandrin induces DNA damage responses in cancer cells by suppressing the expression of Rad51 |
title_short | Oleandrin induces DNA damage responses in cancer cells by suppressing the expression of Rad51 |
title_sort | oleandrin induces dna damage responses in cancer cells by suppressing the expression of rad51 |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5312332/ https://www.ncbi.nlm.nih.gov/pubmed/27449097 http://dx.doi.org/10.18632/oncotarget.10726 |
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