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Oleandrin induces DNA damage responses in cancer cells by suppressing the expression of Rad51

Oleandrin is a monomeric compound extracted from leaves and seeds of Nerium oleander. It had been reported that oleandrin could effectively inhibit the growth of human cancer cells. However, the specific mechanisms of the oleandrin-induced anti-tumor effects remain largely unclear. Genomic instabili...

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Autores principales: Bao, Zhengqiang, Tian, Baoping, Wang, Xiaohui, Feng, Hanrong, Liang, Ye, Chen, Zhihua, Li, Wen, Shen, Huahao, Ying, Songmin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals LLC 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5312332/
https://www.ncbi.nlm.nih.gov/pubmed/27449097
http://dx.doi.org/10.18632/oncotarget.10726
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author Bao, Zhengqiang
Tian, Baoping
Wang, Xiaohui
Feng, Hanrong
Liang, Ye
Chen, Zhihua
Li, Wen
Shen, Huahao
Ying, Songmin
author_facet Bao, Zhengqiang
Tian, Baoping
Wang, Xiaohui
Feng, Hanrong
Liang, Ye
Chen, Zhihua
Li, Wen
Shen, Huahao
Ying, Songmin
author_sort Bao, Zhengqiang
collection PubMed
description Oleandrin is a monomeric compound extracted from leaves and seeds of Nerium oleander. It had been reported that oleandrin could effectively inhibit the growth of human cancer cells. However, the specific mechanisms of the oleandrin-induced anti-tumor effects remain largely unclear. Genomic instability is one of the main features of cancer cells, it can be the combined effect of DNA damage and tumour-specific DNA repair defects. DNA damage plays important roles during tumorigenesis. In fact, most of the current chemotherapy agents were designed to kill cancer cells by inducing DNA damage. In this study, we found that oleandrin was effective to induce apoptosis in cancer cells, and cause rapid DNA damage response, represented by nuclear RPA (Replication Protein A, a single strand DNA binding protein) and γH2AX(a marker for DNA double strand breaks) foci formation. Interestingly, expression of RAD51, a key protein involved in homologous recombination (HR), was suppressed while XRCC1 was up-regulated in oleandrin treated cancer cells. These results suggested that XRCC1 may play a predominant role in repairing oleandrin-induced DNA damage. Collectively, oleandrin may be a potential anti-tumor agent by suppressing the expression of Rad51.
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spelling pubmed-53123322017-03-06 Oleandrin induces DNA damage responses in cancer cells by suppressing the expression of Rad51 Bao, Zhengqiang Tian, Baoping Wang, Xiaohui Feng, Hanrong Liang, Ye Chen, Zhihua Li, Wen Shen, Huahao Ying, Songmin Oncotarget Research Paper Oleandrin is a monomeric compound extracted from leaves and seeds of Nerium oleander. It had been reported that oleandrin could effectively inhibit the growth of human cancer cells. However, the specific mechanisms of the oleandrin-induced anti-tumor effects remain largely unclear. Genomic instability is one of the main features of cancer cells, it can be the combined effect of DNA damage and tumour-specific DNA repair defects. DNA damage plays important roles during tumorigenesis. In fact, most of the current chemotherapy agents were designed to kill cancer cells by inducing DNA damage. In this study, we found that oleandrin was effective to induce apoptosis in cancer cells, and cause rapid DNA damage response, represented by nuclear RPA (Replication Protein A, a single strand DNA binding protein) and γH2AX(a marker for DNA double strand breaks) foci formation. Interestingly, expression of RAD51, a key protein involved in homologous recombination (HR), was suppressed while XRCC1 was up-regulated in oleandrin treated cancer cells. These results suggested that XRCC1 may play a predominant role in repairing oleandrin-induced DNA damage. Collectively, oleandrin may be a potential anti-tumor agent by suppressing the expression of Rad51. Impact Journals LLC 2016-07-20 /pmc/articles/PMC5312332/ /pubmed/27449097 http://dx.doi.org/10.18632/oncotarget.10726 Text en Copyright: © 2016 Bao et al. http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Paper
Bao, Zhengqiang
Tian, Baoping
Wang, Xiaohui
Feng, Hanrong
Liang, Ye
Chen, Zhihua
Li, Wen
Shen, Huahao
Ying, Songmin
Oleandrin induces DNA damage responses in cancer cells by suppressing the expression of Rad51
title Oleandrin induces DNA damage responses in cancer cells by suppressing the expression of Rad51
title_full Oleandrin induces DNA damage responses in cancer cells by suppressing the expression of Rad51
title_fullStr Oleandrin induces DNA damage responses in cancer cells by suppressing the expression of Rad51
title_full_unstemmed Oleandrin induces DNA damage responses in cancer cells by suppressing the expression of Rad51
title_short Oleandrin induces DNA damage responses in cancer cells by suppressing the expression of Rad51
title_sort oleandrin induces dna damage responses in cancer cells by suppressing the expression of rad51
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5312332/
https://www.ncbi.nlm.nih.gov/pubmed/27449097
http://dx.doi.org/10.18632/oncotarget.10726
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