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Mentholation affects the cigarette microbiota by selecting for bacteria resistant to harsh environmental conditions and selecting against potential bacterial pathogens

BACKGROUND: There is a paucity of data regarding the microbial constituents of tobacco products and their impacts on public health. Moreover, there has been no comparative characterization performed on the bacterial microbiota associated with the addition of menthol, an additive that has been used b...

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Autores principales: Chopyk, Jessica, Chattopadhyay, Suhana, Kulkarni, Prachi, Claye, Emma, Babik, Kelsey R., Reid, Molly C., Smyth, Eoghan M., Hittle, Lauren E., Paulson, Joseph N., Cruz-Cano, Raul, Pop, Mihai, Buehler, Stephanie S., Clark, Pamela I., Sapkota, Amy R., Mongodin, Emmanuel F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5312438/
https://www.ncbi.nlm.nih.gov/pubmed/28202080
http://dx.doi.org/10.1186/s40168-017-0235-0
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author Chopyk, Jessica
Chattopadhyay, Suhana
Kulkarni, Prachi
Claye, Emma
Babik, Kelsey R.
Reid, Molly C.
Smyth, Eoghan M.
Hittle, Lauren E.
Paulson, Joseph N.
Cruz-Cano, Raul
Pop, Mihai
Buehler, Stephanie S.
Clark, Pamela I.
Sapkota, Amy R.
Mongodin, Emmanuel F.
author_facet Chopyk, Jessica
Chattopadhyay, Suhana
Kulkarni, Prachi
Claye, Emma
Babik, Kelsey R.
Reid, Molly C.
Smyth, Eoghan M.
Hittle, Lauren E.
Paulson, Joseph N.
Cruz-Cano, Raul
Pop, Mihai
Buehler, Stephanie S.
Clark, Pamela I.
Sapkota, Amy R.
Mongodin, Emmanuel F.
author_sort Chopyk, Jessica
collection PubMed
description BACKGROUND: There is a paucity of data regarding the microbial constituents of tobacco products and their impacts on public health. Moreover, there has been no comparative characterization performed on the bacterial microbiota associated with the addition of menthol, an additive that has been used by tobacco manufacturers for nearly a century. To address this knowledge gap, we conducted bacterial community profiling on tobacco from user- and custom-mentholated/non-mentholated cigarette pairs, as well as a commercially-mentholated product. Total genomic DNA was extracted using a multi-step enzymatic and mechanical lysis protocol followed by PCR amplification of the V3-V4 hypervariable regions of the 16S rRNA gene from five cigarette products (18 cigarettes per product for a total of 90 samples): Camel Crush, user-mentholated Camel Crush, Camel Kings, custom-mentholated Camel Kings, and Newport Menthols. Sequencing was performed on the Illumina MiSeq platform and sequences were processed using the Quantitative Insights Into Microbial Ecology (QIIME) software package. RESULTS: In all products, Pseudomonas was the most abundant genera and included Pseudomonas oryzihabitans and Pseudomonas putida, regardless of mentholation status. However, further comparative analysis of the five products revealed significant differences in the bacterial compositions across products. Bacterial community richness was higher among non-mentholated products compared to those that were mentholated, particularly those that were custom-mentholated. In addition, mentholation appeared to be correlated with a reduction in potential human bacterial pathogens and an increase in bacterial species resistant to harsh environmental conditions. CONCLUSIONS: Taken together, these data provide preliminary evidence that the mentholation of commercially available cigarettes can impact the bacterial community of these products. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s40168-017-0235-0) contains supplementary material, which is available to authorized users.
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spelling pubmed-53124382017-02-24 Mentholation affects the cigarette microbiota by selecting for bacteria resistant to harsh environmental conditions and selecting against potential bacterial pathogens Chopyk, Jessica Chattopadhyay, Suhana Kulkarni, Prachi Claye, Emma Babik, Kelsey R. Reid, Molly C. Smyth, Eoghan M. Hittle, Lauren E. Paulson, Joseph N. Cruz-Cano, Raul Pop, Mihai Buehler, Stephanie S. Clark, Pamela I. Sapkota, Amy R. Mongodin, Emmanuel F. Microbiome Research BACKGROUND: There is a paucity of data regarding the microbial constituents of tobacco products and their impacts on public health. Moreover, there has been no comparative characterization performed on the bacterial microbiota associated with the addition of menthol, an additive that has been used by tobacco manufacturers for nearly a century. To address this knowledge gap, we conducted bacterial community profiling on tobacco from user- and custom-mentholated/non-mentholated cigarette pairs, as well as a commercially-mentholated product. Total genomic DNA was extracted using a multi-step enzymatic and mechanical lysis protocol followed by PCR amplification of the V3-V4 hypervariable regions of the 16S rRNA gene from five cigarette products (18 cigarettes per product for a total of 90 samples): Camel Crush, user-mentholated Camel Crush, Camel Kings, custom-mentholated Camel Kings, and Newport Menthols. Sequencing was performed on the Illumina MiSeq platform and sequences were processed using the Quantitative Insights Into Microbial Ecology (QIIME) software package. RESULTS: In all products, Pseudomonas was the most abundant genera and included Pseudomonas oryzihabitans and Pseudomonas putida, regardless of mentholation status. However, further comparative analysis of the five products revealed significant differences in the bacterial compositions across products. Bacterial community richness was higher among non-mentholated products compared to those that were mentholated, particularly those that were custom-mentholated. In addition, mentholation appeared to be correlated with a reduction in potential human bacterial pathogens and an increase in bacterial species resistant to harsh environmental conditions. CONCLUSIONS: Taken together, these data provide preliminary evidence that the mentholation of commercially available cigarettes can impact the bacterial community of these products. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s40168-017-0235-0) contains supplementary material, which is available to authorized users. BioMed Central 2017-02-15 /pmc/articles/PMC5312438/ /pubmed/28202080 http://dx.doi.org/10.1186/s40168-017-0235-0 Text en © The Author(s). 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Chopyk, Jessica
Chattopadhyay, Suhana
Kulkarni, Prachi
Claye, Emma
Babik, Kelsey R.
Reid, Molly C.
Smyth, Eoghan M.
Hittle, Lauren E.
Paulson, Joseph N.
Cruz-Cano, Raul
Pop, Mihai
Buehler, Stephanie S.
Clark, Pamela I.
Sapkota, Amy R.
Mongodin, Emmanuel F.
Mentholation affects the cigarette microbiota by selecting for bacteria resistant to harsh environmental conditions and selecting against potential bacterial pathogens
title Mentholation affects the cigarette microbiota by selecting for bacteria resistant to harsh environmental conditions and selecting against potential bacterial pathogens
title_full Mentholation affects the cigarette microbiota by selecting for bacteria resistant to harsh environmental conditions and selecting against potential bacterial pathogens
title_fullStr Mentholation affects the cigarette microbiota by selecting for bacteria resistant to harsh environmental conditions and selecting against potential bacterial pathogens
title_full_unstemmed Mentholation affects the cigarette microbiota by selecting for bacteria resistant to harsh environmental conditions and selecting against potential bacterial pathogens
title_short Mentholation affects the cigarette microbiota by selecting for bacteria resistant to harsh environmental conditions and selecting against potential bacterial pathogens
title_sort mentholation affects the cigarette microbiota by selecting for bacteria resistant to harsh environmental conditions and selecting against potential bacterial pathogens
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5312438/
https://www.ncbi.nlm.nih.gov/pubmed/28202080
http://dx.doi.org/10.1186/s40168-017-0235-0
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