An efficient system for the generation of marked genetic mutants in members of the genus Burkholderia

To elucidate the function of a gene in bacteria it is vital that targeted gene inactivation (allelic replacement) can be achieved. Allelic replacement is often carried out by disruption of the gene of interest by insertion of an antibiotic-resistance marker followed by subsequent transfer of the mut...

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Detalles Bibliográficos
Autores principales: Shastri, Sravanthi, Spiewak, Helena L., Sofoluwe, Aderonke, Eidsvaag, Vigdis A., Asghar, Atif H., Pereira, Tyrone, Bull, Edward H., Butt, Aaron T., Thomas, Mark S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Academic Press 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5312678/
https://www.ncbi.nlm.nih.gov/pubmed/27825973
http://dx.doi.org/10.1016/j.plasmid.2016.11.002

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5312678/
https://www.ncbi.nlm.nih.gov/pubmed/27825973
http://dx.doi.org/10.1016/j.plasmid.2016.11.002

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